Rowntree C I, Bland B H
Brain Res. 1986 Jan 1;362(1):98-113. doi: 10.1016/0006-8993(86)91403-4.
Microinfusions of cholinergic agents were made in various sites in the dorsal hippocampal formation of urethane anaesthetized rats. Infusions of eserine or carbachol elicited hippocampal theta activity when made in areas containing high levels of cholinergic markers: the stratum oriens and radiatum of the CA1 and CA3, the stratum moleculare and stratum granulosum of the dentate gyrus and the infragranular region of the hilus. Subsequent infusions of atropine sulfate antagonized the theta activity. Control infusions of equal volumes of saline in active sites were without effect. Infusions of eserine or carbachol in the vicinity of the hippocampal fissure, the stratum lacunosum/moleculare of the CA1 or CA3, in the deep regions of the hilus, and in the lateral ventricle and overlying neocortex, were also without effect. Furthermore, in active sites, the latency to onset of theta and subsequent theta frequency, were both directly related to the total amount of carbachol infused. Thus, areas in which theta could be elicited with a cholinergic agonist (carbachol), or an anticholinesterase (eserine) and antagonized with atropine, were found to correspond well to areas previously found to contain a high density of cholinoceptive neurons, using autoradiographic and immunohistochemical techniques. These results provide further support for the involvement of acetylcholine as a neurotransmitter in the generation of type 2 theta in the hippocampal formation.
在氨基甲酸乙酯麻醉的大鼠背侧海马结构的不同部位进行胆碱能药物的微量注射。当在含有高水平胆碱能标记物的区域注射毒扁豆碱或卡巴胆碱时，可诱发海马θ波活动，这些区域包括CA1和CA3的海马下托和辐射层、齿状回的分子层和颗粒层以及海马回的颗粒下层。随后注射硫酸阿托品可拮抗θ波活动。在活跃部位注射等量生理盐水作为对照则无此效应。在海马裂附近、CA1或CA3的腔隙/分子层、海马回深部区域、侧脑室及覆盖的新皮质注射毒扁豆碱或卡巴胆碱也无效应。此外，在活跃部位，θ波起始潜伏期及随后的θ波频率均与注入的卡巴胆碱总量直接相关。因此，发现能被胆碱能激动剂（卡巴胆碱）或抗胆碱酯酶（毒扁豆碱）诱发并被阿托品拮抗θ波的区域，与先前利用放射自显影和免疫组织化学技术发现的含有高密度胆碱能感受神经元的区域非常吻合。这些结果进一步支持了乙酰胆碱作为神经递质参与海马结构中2型θ波产生的观点。
