Lawson V H, Bland B H
Department of Psychology, University of Calgary, Alberta, Canada.
Exp Neurol. 1993 Mar;120(1):132-44. doi: 10.1006/exnr.1993.1047.
The role of the septohippocampal pathway in the regulation of hippocampal field activity and behavior was assessed using the technique of intraseptal microinfusion of carbachol, atropine, and procaine. The effects of these manipulations were assessed by comparing them with the effects of intraseptal saline control infusions on spontaneously occurring motor behaviors and their correlated hippocampal field activities. In control conditions hippocampal theta field activity, with up to 40% of the total power concentrated in a 1-Hz band around the peak frequency, was recorded only during type 1 voluntary motor behaviors. Hippocampal large-amplitude irregular activity was recorded during type 2 automatic motor behaviors and during immobility. Microinfusion of carbachol into the MS/vDBB (medial septum/vertical limb of the diagonal band of Broca) resulted in continuous theta field activity regardless of what type of motor behavior the animal performed or whether it was immobile. However, under these conditions the performance of a voluntary movement consistently resulted in an upward shift of theta frequency, demonstrating that the frequency of carbachol-elicited type 2 theta can be modulated by the behavioral activation of type 1 theta. The subsequent infusion of atropine abolished the carbachol-elicited theta, while that occurring during type 1 voluntary movement was preserved. In contrast to the selective blockade of carbachol-elicited theta by atropine, procaine suppression of the MS/vDBB abolished both spontaneous movement-related (type 1) theta and carbachol-elicited (type 2) theta. During the postprocaine period theta frequency recovered rapidly in contrast to theta amplitude (power). This result applied to the recovery of both the coactivated type 1 and type 2 theta occurring during spontaneous movement and the carbachol-elicited type 2 theta alone. The behavioral results were discussed within the context of Bland's (Prog. Neurobiol. 26, 1-54, 1986) sensorimotor model which posits that hippocampal theta activity recorded during voluntary movement represents the coactivation of a cholinergically mediated sensory processing component and a feedback (possibly serotonergic) motor component.
运用向中隔内微量注射卡巴胆碱、阿托品和普鲁卡因的技术，评估了中隔 - 海马通路在调节海马场活动及行为中的作用。通过将这些操作的效果与向中隔内注射生理盐水作为对照时对自发运动行为及其相关海马场活动的影响进行比较，来评估这些操作的效果。在对照条件下，海马θ场活动仅在1型自主运动行为期间被记录到，其总功率的高达40%集中在峰值频率周围的1赫兹频段内。在2型自动运动行为期间及静止不动时记录到海马大振幅不规则活动。向内侧隔核/布罗卡斜带垂直支（MS/vDBB）微量注射卡巴胆碱会导致持续的θ场活动，无论动物进行何种类型的运动行为或是否静止不动。然而，在这些条件下，自主运动的执行始终会导致θ频率向上偏移，表明1型θ的行为激活可调节卡巴胆碱诱发型2 θ的频率。随后注射阿托品可消除卡巴胆碱诱发的θ，但1型自主运动期间出现的θ得以保留。与阿托品对卡巴胆碱诱发θ的选择性阻断相反，普鲁卡因对MS/vDBB的抑制作用消除了与自发运动相关的（1型）θ和卡巴胆碱诱发的（2型）θ。在注射普鲁卡因后的时期，与θ振幅（功率）相比，θ频率迅速恢复。这一结果适用于自发运动期间共同激活的1型和2型θ以及单独的卡巴胆碱诱发的2型θ的恢复。在布兰德（《神经生物学进展》26卷，第1 - 54页，1986年）的感觉运动模型背景下讨论了行为学结果，该模型假定在自主运动期间记录到的海马θ活动代表胆碱能介导的感觉处理成分和反馈（可能是5 - 羟色胺能）运动成分的共同激活。
