Siebler M, Schmidt H
Brain Res. 1986 Jan 8;362(2):299-307. doi: 10.1016/0006-8993(86)90454-3.
Experiments were made to induce action potentials in innervated slow muscle fibers of Rana temporaria. Drugs were applied to the sciatic nerve or its spinal roots by one of the following methods: implantation of a silastic cuff containing tetrodotoxin (TTX); subepineural injection of TTX combined with subcutaneous injections of vincristine; and epineural application of colchicine or vincristine. Twelve to 50 days later slow fibers responded to direct or indirect stimulation with an action potential. A substantial number of these fibers were innervated by 2 or 3 slow motor axons. It is concluded that these effects of TTX and colchicine (vincristine) are due to inhibition of the axoplasmic transport of an unidentified substance. The results support the hypothesis that the inexcitability of normal slow fibers is due to a 'trophic' effect of their motor axons.
实验旨在诱导林蛙受神经支配的慢肌纤维产生动作电位。通过以下方法之一将药物应用于坐骨神经或其脊髓神经根：植入含有河豚毒素（TTX）的硅橡胶套；神经外膜下注射TTX并皮下注射长春新碱；以及在神经外膜应用秋水仙碱或长春新碱。12至50天后，慢肌纤维对直接或间接刺激产生动作电位反应。这些纤维中有相当一部分由2至3条慢运动轴突支配。得出的结论是，TTX和秋水仙碱（长春新碱）的这些作用是由于对一种未鉴定物质的轴浆运输的抑制。结果支持了这样的假说，即正常慢肌纤维的兴奋性缺失是由于其运动轴突的“营养”作用。
