Jeyapriya Marytresa S, Kumar Sathish M, Nirmal Madhavan R
Oral Pathology and Microbiology, Karpaga Vinayaga Institute of Dental Sciences, Chengalpet, IND.
Oral and Maxillofacial Pathology, Rajah Muthiah Dental College and Hospital, Chidambaram, IND.
Cureus. 2024 Sep 20;16(9):e69790. doi: 10.7759/cureus.69790. eCollection 2024 Sep.
Background Oral squamous cell carcinoma (OSCC) accounts for the majority of oral cancers globally. It is characterized by metastasis, poor prognosis, high recurrence rate, and poor five-year survival rate due to late detection or diagnosis at an advanced stage. Novel biomarkers that can predict the prognosis of patients with OSCC are needed to improve survival. Lysosome-associated membrane protein-3 (LAMP3) glycoprotein, a member of the LAMP protein family, is a molecular marker for mature dendritic cells. LAMP3 expression has been correlated with unfavorable prognosis in patients with various cancers. Few studies have examined the relationship between LAMP3 and clinicopathological parameters in OSCC. This study aims to analyze the immunohistochemical expression of LAMP3 in OSCC and its relationship with clinicopathological characteristics. Methodology In this study, 75 formalin-fixed, paraffin-embedded samples of cases diagnosed with primary OSCC were obtained and immunostained with LAMP3 antibody. Its expression was compared with clinicopathological parameters such as age, sex, tobacco and alcohol consumption habits, differentiation, TNM staging, tumor location, lymph node metastasis, lymphovascular invasion, perineural invasion, and pattern of invasion. Results Higher LAMP3 expression was highly significantly associated with the TNM stage (p = 0.001). High expression of LAMP3 was significantly associated with T stage (p = 0.002) and lymph node metastasis (p = 0.002). All poorly differentiated OSCC cases (n = 2, 100%) showed a high expression of LAMP3. Conclusions High LAMP3 expression and its significant association with TNM stage, T stage, and lymph node metastasis suggest a potential role for LAMP3 in OSCC carcinogenesis. High LAMP3 expression in poorly differentiated OSCC might indicate that it plays a pivotal role in oncogenic cell transformation. Our results indicate that LAMP3 may be a predictive marker for poor prognosis in OSCC.
口腔鳞状细胞癌（OSCC）在全球口腔癌中占大多数。其特点是会发生转移、预后差、复发率高，且由于晚期才被发现或诊断，五年生存率较低。需要新的生物标志物来预测OSCC患者的预后，以提高生存率。溶酶体相关膜蛋白3（LAMP3）糖蛋白是LAMP蛋白家族的成员，是成熟树突状细胞的分子标志物。LAMP3的表达与多种癌症患者的不良预后相关。很少有研究探讨LAMP3与OSCC临床病理参数之间的关系。本研究旨在分析LAMP3在OSCC中的免疫组化表达及其与临床病理特征的关系。
本研究获取了75例经福尔马林固定、石蜡包埋的原发性OSCC确诊病例样本，并用LAMP3抗体进行免疫染色。将其表达与年龄、性别、烟酒消费习惯、分化程度、TNM分期、肿瘤位置、淋巴结转移、淋巴管浸润、神经周围浸润和浸润模式等临床病理参数进行比较。
LAMP3高表达与TNM分期高度显著相关（p = 0.001）。LAMP3高表达与T分期（p = 0.002）和淋巴结转移（p = 0.002）显著相关。所有低分化OSCC病例（n = 2，100%）均显示LAMP3高表达。
LAMP3高表达及其与TNM分期、T分期和淋巴结转移的显著关联表明LAMP3在OSCC致癌过程中可能发挥作用。低分化OSCC中LAMP3高表达可能表明它在致癌细胞转化中起关键作用。我们的结果表明LAMP3可能是OSCC预后不良的预测标志物。
