印度尼西亚亚齐省加约高原生长的乙醇提取物的α-葡萄糖苷酶抑制作用。

Alpha-Glucosidase Inhibitory Effect of Ethanol Extract Growing in Gayo Highland, Aceh Province, Indonesia.

机构信息

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh, Aceh, 23111, Indonesia.

Clinical Laboratory, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Aceh, 23111, Indonesia.

出版信息

F1000Res. 2024 Sep 4;13:489. doi: 10.12688/f1000research.149029.2. eCollection 2024.

DOI:10.12688/f1000research.149029.2

PMID:39429642

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11487234/

Abstract

BACKGROUND

The prevalence of diabetes mellitus (DM) is increasing overtime, potentially leading to various severe health complications and mortality. Despite therapeutic agents have currently been developed, unexpected adverse effects are inevitable. Hence, safe and effective medications such as those of plant origin are critical to prevent unexpected complication in DM sufferers. has been widely used as spice and traditional medicine to treat diabetes in Aceh Province, Indonesia. However, study regarding α-glucosidase inhibitory effect of growing in Gayo highlands, Aceh, Indonesia, is completely lacking. The aim of this study was to evaluate α-glucosidase inhibitory effect of ethanol extracts (EEEE) growing in Gayo highlands, Aceh Province, Indonesia.

METHODS

Antioxidant activity was determined using DPPH procedure, whereas α-glucosidase inhibition assay was carried out using spectrophotometric method. Data analysis was performed using One-Way Analysis of Variance (ANOVA), followed by Duncan's multiple range test at α=0.05.

RESULTS

Phytochemical analysis revealed the presence of total phenolic (TPC), total flavonoid (TFC), and total tannin (TTC) content in all plant parts, in which the highest TPC was found in the stem (158.38 GAE/g), whereas the highest TFC and TTC was obtained in the rhizome extracts. The extract of fruit showed the strongest antioxidant activities, followed by the stem and leaf, with IC of 2.381 μg/mL, 6.966 μg/mL, and 19.365 μg/mL, respectively. All extracts revealed a significant inhibitory activity against α-glucosidase at the concentration of 500 μg/mL, in which the stem extract showed the most effective α-glucosidase inhibitory effect with IC value of 5.15 μg/mL, suggesting its promising potential as antidiabetic agent.

CONCLUSIONS

This study highlights potency as a novel source of antioxidant and natural antidiabetic compounds that are useful for the prevention and treatment of diabetes.

摘要

背景

糖尿病（DM）的患病率呈上升趋势，可能导致各种严重的健康并发症和死亡率。尽管目前已经开发出治疗药物，但不可避免地会出现意外的不良反应。因此，像植物来源的那样安全有效的药物对于预防 DM 患者的意外并发症至关重要。在印度尼西亚亚齐省，被广泛用作香料和传统药物来治疗糖尿病。然而，关于在印度尼西亚亚齐哥多高原生长的的 α-葡萄糖苷酶抑制作用的研究完全缺乏。本研究旨在评估印度尼西亚亚齐哥多高原生长的的乙醇提取物（EEEE）的 α-葡萄糖苷酶抑制作用。

方法

使用 DPPH 程序测定抗氧化活性，使用分光光度法测定 α-葡萄糖苷酶抑制活性。使用单因素方差分析（ANOVA）进行数据分析，然后使用 Duncan 多重范围检验在 α=0.05 时进行检验。

结果

植物化学分析表明，所有植物部位均存在总酚（TPC）、总黄酮（TFC）和总单宁（TTC）含量，其中茎部的 TPC 含量最高（158.38 GAE/g），而 TFC 和 TTC 含量最高的是根茎提取物。果实提取物表现出最强的抗氧化活性，其次是茎和叶，IC 分别为 2.381 μg/mL、6.966 μg/mL 和 19.365 μg/mL。所有提取物在 500 μg/mL 浓度下均对 α-葡萄糖苷酶表现出显著的抑制活性，其中茎提取物对 α-葡萄糖苷酶的抑制作用最有效，IC 为 5.15 μg/mL，表明其作为抗糖尿病药物具有潜在的应用前景。