Zekeya Never, Ibrahim Mariam, Mamiro Bertha, Ndossi Humphrey, Kilonzo Mhuji, Mkangara Mwanaisha, Chacha Musa, Chilongola Jaffu, Kideghesho Jafari
Department of Wildlife Management, College of African Wildlife Management, CAWM, P.O. Box 3031, Mweka, Moshi, Kilimanjaro, Tanzania.
Tanzania Industrial Research and Development Organization (TIRDO), P.O. Box 23235, Msasani, Dar essalaam, Tanzania.
Saudi J Biol Sci. 2022 Jun;29(6):103273. doi: 10.1016/j.sjbs.2022.03.023. Epub 2022 Mar 23.
Chronic diseases including cardiovascular, diabetes and cancer persist for a long time in the course of treatment affecting health and are currently the cause of many deaths. In most cases, the treatment of chronic infectious diseases especially Tuberculosis relies on conventional drugs which are currently becoming fruitless due to drug resistance and unpredicted complications in course of treatment. However, herbal medicines have for a long time been used in prevention and treatment of chronic diseases including asthma and heart diseases in Africa. In this study, we extracted metabolites and screened for active compounds with potential free radical scavenging and pharmacological activities from the plant commonly used in traditional medicine in Tanzania. root, stembark and leaf were air dried, sequentially extracted in various solvents including petroleum ether, dichloromethane, ethylacetate and methanol to yield extracts and fractions. The extracts and fractions were tested for the presence of several metabolites and antioxidant activity. The analysis of chemical compounds from resultant extracts was done by GC-MS for non-polar factions and LC-MS/MC for moderate polar extracts.High amount of phenolic acid, flavonoids and tannin were identified in ethylacetate fraction compared to ethanol, dichloromethane and petroleum ether. The GC-MS analysis of petroleum ether extract of stem back yielded twelve (12) compounds with varying composition. The most abundant compounds were 2-Butenoic acid, 3-methyl-, ethyl ester comprising 33.8%, n-Hexadecanoic acid comprising 16.7% and Ethanolpentamethyl- yielded in 16.7%.The LC-MS/MS analysis of Ethyl acetate fractions yielded 20 compounds including; Mangiferin and Isoquercitin were abundant in leaves, stembark and roots. Lastly, ethyl vanillate was identified in both roots and leaves whereas Quercitrin and 7,8-Dimethoxycoumarin were found in stembark and root.These findings indicated that is rich in phenolic compounds ranging from phenolic acids, flavonoids and coumarin that possess high antioxidant and pharmacological properties potential for treatment of chronic diseases.
包括心血管疾病、糖尿病和癌症在内的慢性疾病在治疗过程中会长期存在,影响健康,并且目前是许多人死亡的原因。在大多数情况下,慢性传染病尤其是结核病的治疗依赖于传统药物,由于耐药性和治疗过程中不可预测的并发症,目前这些药物正变得无效。然而,长期以来,草药一直被用于非洲预防和治疗包括哮喘和心脏病在内的慢性疾病。在本研究中,我们从坦桑尼亚传统医学常用的植物中提取代谢物,并筛选具有潜在自由基清除和药理活性的活性化合物。根、茎皮和叶经风干后,依次用包括石油醚、二氯甲烷、乙酸乙酯和甲醇在内的各种溶剂提取,得到提取物和馏分。对提取物和馏分进行了几种代谢物的存在和抗氧化活性测试。通过气相色谱 - 质谱联用仪(GC-MS)对非极性馏分进行分析,通过液相色谱 - 质谱联用仪(LC-MS/MS)对中等极性提取物进行分析。与乙醇、二氯甲烷和石油醚相比,乙酸乙酯馏分中鉴定出大量的酚酸、黄酮类化合物和单宁。茎皮石油醚提取物的气相色谱 - 质谱联用仪分析产生了十二种(12)成分各异的化合物。含量最高的化合物是2-丁烯酸,3-甲基 - ,乙酯占33.8%,正十六烷酸占16.7%,乙醇五甲基 - 占16.7%。乙酸乙酯馏分的液相色谱 - 质谱联用仪分析产生了20种化合物,包括:芒果苷和异槲皮苷在叶、茎皮和根中含量丰富。最后,在根和叶中均鉴定出香草酸乙酯,而在茎皮和根中发现了槲皮苷和7,8-二甲氧基香豆素。这些发现表明,该植物富含酚类化合物,包括酚酸、黄酮类化合物和香豆素,这些化合物具有高抗氧化和药理特性,具有治疗慢性疾病的潜力。