Hofstädter F, Delgado R, Jakse G, Judmaier W
Cancer. 1986 Jan 15;57(2):356-61. doi: 10.1002/1097-0142(19860115)57:2<356::aid-cncr2820570228>3.0.co;2-7.
Urothelial cells were pepsin-extracted from paraffin-embedded specimens taken from human nontumorous bladder mucosa, dysplasia, and carcinoma in situ. After Feulgen staining for DNA, nuclei were measured with an integrating microdensitometer. The measurements show that normal urothelium consists mostly of diploid nuclei. Dysplasia means that there is a predominance of tetraploid DNA values, whereas carcinoma in situ is characterized by a high percentage of aneuploid cells. In both dysplasia and carcinoma in situ there is a considerable percentage of diploid nuclei. Thus, DNA cytophotometry can be used for standardization of preneoplastic and early stages of tumor development in bladder cancer.
从取自人非肿瘤性膀胱黏膜、发育异常及原位癌的石蜡包埋标本中用胃蛋白酶提取尿路上皮细胞。对DNA进行福尔根染色后，用积分光密度计测量细胞核。测量结果表明，正常尿路上皮主要由二倍体细胞核组成。发育异常意味着四倍体DNA值占优势，而原位癌的特征是非整倍体细胞的比例很高。在发育异常和原位癌中，二倍体细胞核的比例都相当可观。因此，DNA细胞光度测定法可用于膀胱癌肿瘤发生前及肿瘤发展早期阶段的标准化。
