Blaabjerg O, Hyltoft Petersen P
Scand J Clin Lab Invest. 1979 Dec;39(8):751-7. doi: 10.3109/00365517909108167.
In order to investigate the consequences of presence of aggregates in human albumin standards, pools of monomer, dimer and polymer albumin were prepared and quantitated by three total-protein methods (biuret, Folin-Lowry and spectrophotometry at 279 nm) and by four different albumin methods (dye-binding by bromcresol green, electroimmunoassay, radial immunodiffusion and automated immunoprecipitation). Biuret was chosen as the reference method and the monomer was used as the standard in all methods. Both the total-protein and albumin methods gave values for aggregated albumin different from the biuret values. The maximal bias occurred in radial immunodiffusion where quantitation of the dimer and polymer pools gave only 67% and 48% of the biuret values, respectively. In five commercial albumin preparations investigated, the content of di- and polymer varied from 3 to 34%. Uncritical use of albumin preparations in standardization may thus introduce bias in the measurements.
为了研究人白蛋白标准品中聚集体存在的后果，制备了单体、二聚体和多聚体白蛋白池，并通过三种总蛋白方法（双缩脲法、福林-劳里法和279nm分光光度法）以及四种不同的白蛋白方法（溴甲酚绿染料结合法、电免疫分析法、放射免疫扩散法和自动免疫沉淀法）进行定量。选择双缩脲法作为参考方法，在所有方法中均以单体作为标准品。总蛋白方法和白蛋白方法得出的聚集白蛋白值均与双缩脲法的值不同。最大偏差出现在放射免疫扩散法中，二聚体和多聚体池的定量分别仅为双缩脲法值的67%和48%。在所研究的五种商业白蛋白制剂中，二聚体和多聚体的含量在3%至34%之间变化。因此，在标准化过程中不加区分地使用白蛋白制剂可能会在测量中引入偏差。
