Fu Jun, Tian Chuanchuan, Wan Xuchun, Hu Ruibin, Yu Jiaojun, Zhang Jialiang, Wang Shuzhen
College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang, Hubei Province, 438000, China.
AoB Plants. 2024 Oct 14;16(5):plae053. doi: 10.1093/aobpla/plae053. eCollection 2024 Oct.
Systems-wide understanding of gene expression profile regulating flower colour formation in Planchon is insufficient. In this research, integration analysis of ribonucleic acid (RNA)omics and microRNAome were performed to reveal the molecular mechanism of flower colour formation in three varieties with red, pink and crimson flowers, respectively. Totally, 3129, 5755 and 5295 differentially expressed gene (DEG)s were identified through comparative transcriptome analysis between 'Red variety' and 'Pink variety' (1507 up-regulated and 1622 down-regulated), 'Red variety' and 'Crimson variety' (2148 up-regulated 3607 down-regulated), as well as 'Pink variety' and 'Crimson variety' (2089 up-regulated and 3206 down-regulated), which were involved in processes of 'catalytic activity', 'binding', 'metabolic process' and 'cellular process', as well as pathways of 'metabolic pathways', 'biosynthesis of secondary metabolites', 'plant-pathogen interaction' and 'phenylpropanoid biosynthesis'. A total of 215 miRNAs, containing 153 known miRNAs belonging to 57 families and 62 novel miRNA, were involved in flower colour formation. In particular, 55 miRNAs were significantly differently expressed. Based on miRNA-mRNA regulatory network, ath-miR5658 could affect the synthesis of pelargonidin, cyanidin and delphinidin through downregulating accumulation of anthocyanidin 3-O-glucosyltransferase; ath-miR868-3p could regulate isoflavonoid biosynthesis through downregulating expression of CYP81E1/E7; ath-miR156g regulated the expression of flavonoid 3',5'-hydroxylase; and ath-miR829-5p regulated flavonol synthasein flavonoid biosynthesis process. This research will provide important roles in breeding new varieties with rich flower colour.
目前对普兰雄（Planchon）中调控花色形成的基因表达谱缺乏全系统的了解。在本研究中，分别对红色、粉色和深红色花朵的三个品种进行了核糖核酸（RNA）组学和微小RNA组的整合分析，以揭示花色形成的分子机制。通过比较转录组分析，在“红色品种”与“粉色品种”（1507个上调和1622个下调）、“红色品种”与“深红色品种”（2148个上调和3607个下调）以及“粉色品种”与“深红色品种”（2089个上调和3206个下调）之间，共鉴定出3129个、5755个和5295个差异表达基因（DEG），这些基因参与了“催化活性”“结合”“代谢过程”和“细胞过程”，以及“代谢途径”“次生代谢物的生物合成”“植物 - 病原体相互作用”和“苯丙烷类生物合成”等途径。共有215个miRNA参与了花色形成，其中包括属于57个家族的153个已知miRNA和62个新的miRNA。特别是，有55个miRNA的表达存在显著差异。基于miRNA - mRNA调控网络，ath - miR5658可通过下调花青素3 - O - 葡萄糖基转移酶的积累来影响天竺葵色素、矢车菊色素和飞燕草色素的合成；ath - miR868 - 3p可通过下调CYP81E1/E7的表达来调节异黄酮生物合成；ath - miR156g调控类黄酮3'，5'-羟化酶的表达；ath - miR829 - 5p在类黄酮生物合成过程中调控黄酮醇合酶。本研究将为培育花色丰富的新品种发挥重要作用。
