College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang 438000, China.
Department of Library, Huanggang Normal University, Huanggang 438000, China.
Genes (Basel). 2024 Aug 7;15(8):1041. doi: 10.3390/genes15081041.
Planchon is an important ornamental species in the northern hemisphere. Flower color is an important objective of breeding programs. However, information on anthocyanin synthesis in is limited. In this research, the regulatory mechanism of anthocyanin biosynthesis in was performed through the integrated analysis of metabolome and RNA-seq. A total of 805 and 513 metabolites were screened by positive and negative ionization modes, respectively, In total, 79 flavonoids contained seven anthocyanidins, 42 flavanones, 10 flavans, 13 flavones, and seven flavonols. Methylated and glycosylated derivatives took up the most. Differentially accumulated metabolites were mainly involved in "flavone and flavonol biosynthesis", "cyanoamino acid metabolism", "pyrimidine metabolism", and "phenylalanine metabolism" pathways. For flavonoid biosynthesis, different expression of , , , , , , , , and genes ultimately led to different accumulations of quercetin, myricetin, cyanidin, and eriodictyol. In flavone and flavonol biosynthesis pathway, differential expression of , and genes led to the differential accumulation of quercetin, isovitexin, and laricitrin. This research will provide a biochemical basis for further modification of flower color and genetic breeding in and related species.
滇丁香是北半球重要的观赏植物。花色是品种选育的重要目标之一。然而，有关滇丁香中花色形成的分子机制的研究却很少。本研究通过代谢组学和 RNA-seq 联合分析，对滇丁香花色形成的调控机制进行了研究。正、负离子模式分别筛选到 805 种和 513 种代谢物，共鉴定出 79 种类黄酮，包含 7 种花色素苷、42 种黄烷酮、10 种二氢黄酮醇、13 种黄酮和 7 种黄酮醇。其中，甲基化和糖基化衍生物占比最高。差异积累代谢物主要参与“黄酮和黄酮醇生物合成”、“氰基氨基酸代谢”、“嘧啶代谢”和“苯丙氨酸代谢”途径。在类黄酮生物合成途径中，、、、、、、、和 基因的不同表达最终导致了槲皮素、杨梅素、矢车菊素和圣草酚的不同积累。在黄酮和黄酮醇生物合成途径中，、和 基因的差异表达导致了槲皮素、异荭草素和黎芦醇的差异积累。本研究为进一步对滇丁香花色进行修饰以及对相关丁香属植物进行遗传改良提供了生化基础。
