建立多重实时 PCR 方法同时检测侵袭性真菌病中的常见真菌病原体。
Development of multiplex real-time PCR for simultaneous detection of common fungal pathogens in invasive mycoses.
机构信息
Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.
School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.
出版信息
PeerJ. 2024 Oct 17;12:e18238. doi: 10.7717/peerj.18238. eCollection 2024.
BACKGROUND
Fungi are common opportunistic pathogens that pose a significant threat to immunocompromised patients, particularly when late detection occurs.
METHODS
In this study a multiplex real-time PCR has been developed for simultaneous detection of common fungal pathogens associated with invasive mycoses in a diagnostic setting.
RESULTS
The specificity of the assay was rigorously tested on 40 types of organisms ( = 65), demonstrating 100% specificity. The limit of detection was determined to be 100 pg/μl (10 copies/μl), achievable within a rapid 3-h timeframe. The PCR assay efficiency exhibited a range between 89.77% and 104.30% for each target organism, with linearity falling between 0.9780 and 0.9983.
CONCLUSION
This multiplex real-time PCR assay holds promise for enhancing the timely and accurate diagnosis of invasive mycoses, particularly in immunocompromised patient populations.
背景
真菌是常见的机会性病原体，对免疫功能低下的患者构成重大威胁，尤其是在晚期检测时。
方法
本研究开发了一种多重实时 PCR 方法，用于在诊断环境中同时检测与侵袭性真菌感染相关的常见真菌病原体。
结果
该检测方法在 40 种（=65）生物体上进行了严格的特异性测试，显示出 100%的特异性。检测限确定为 100pg/μl（10 拷贝/μl），可在 3 小时内快速达到。PCR 检测方法的效率对于每个目标生物体在 89.77%至 104.30%之间，线性度在 0.9780 和 0.9983 之间。
结论
这种多重实时 PCR 检测方法有望提高侵袭性真菌感染的及时和准确诊断，特别是在免疫功能低下的患者人群中。
Zotero 插件