School of Public Health, Anhui Medical University, Anhui, China.
Department for Disinfection and Infection Control, Chinese PLA Center for Disease Control and Prevention, Beijing, China.
Microbiol Spectr. 2024 Sep 3;12(9):e0063424. doi: 10.1128/spectrum.00634-24. Epub 2024 Jul 30.
Filamentous fungi present significant health hazards to immunocompromised individuals globally; however, the prompt and precise identification of them during infection remains challenging. In this study, a TaqMan probe-based multiplex real-time PCR (M-qPCR) assay was developed to detect simultaneously the target genes of four important pathogenic filamentous fungi: gene of gene of spp, mitochondrial gene of Mucorales, and gene of . In this M-qPCR assay, the limit of detection (LoD) to all four kinds of fungi was 100 copies and the correlation coefficients (R) were above 0.99. The specificity of this assay is 100%, and the minimum detection limit is 100 copies/reaction. In conclusion, an M-qPCR detection assay was well established with high specificity and sensitivity for rapid and simultaneous detection on four important filamentous fungi in the clinic.
World Health Organization developed the first fungal priority pathogens list (WHO FPPL) in 2022. , Mucorales, spp., and spp. are the four types of pathogenic fungi with filamentous morphology in the critical priority group and high priority group of WHO FPPL. These four filamentous fungal infections have become more common and severe in immunocompromised patients with the increase in susceptible populations in recent decades, which resulted in a substantial burden on the public health system. However, prompt and precise identification of them during infection remains challenging. Our study established successfully a TaqMan probe-based multiplex real-time qPCR assay for four clinically important filamentous fungi, , spp., Mucorales, and with high sensitivity and specificity, which shows promising potential for prompt and precise diagnosis against fungal infection.
丝状真菌对全球免疫功能低下的个体构成重大健康危害；然而，在感染过程中及时准确地识别它们仍然具有挑战性。在本研究中，开发了一种基于 TaqMan 探针的多重实时 PCR（M-qPCR）检测方法，用于同时检测四种重要致病性丝状真菌的靶基因：基因、基因、属种、穆尔科菌属的线粒体基因和属种的基因。在该 M-qPCR 检测中，四种真菌的检测限（LoD）均为 100 拷贝，相关系数（R）均高于 0.99。该检测方法的特异性为 100%，最小检测限为 100 拷贝/反应。总之，建立了一种 M-qPCR 检测方法，具有高特异性和灵敏度，可用于临床中快速同时检测四种重要的丝状真菌。
世界卫生组织于 2022 年制定了第一个真菌优先病原体清单（WHO FPPL）。曲霉属、穆尔科菌属、属种和属种是世卫组织 FPPL 关键优先组和高优先组中具有丝状形态的四种致病性真菌。在过去几十年中，免疫功能低下患者中这些四种丝状真菌感染变得更加普遍和严重，这给公共卫生系统带来了巨大负担。然而，在感染过程中及时准确地识别它们仍然具有挑战性。我们的研究成功地建立了一种基于 TaqMan 探针的多重实时 qPCR 检测方法，用于检测四种临床重要的丝状真菌，包括曲霉属、穆尔科菌属、属种和属种，具有高灵敏度和特异性，这表明该方法具有快速准确诊断真菌感染的潜力。
