Aldaz C M, Conti C J, Klein-Szanto A J, Slaga T J
Cancer Genet Cytogenet. 1986 Feb 15;20(3-4):223-9. doi: 10.1016/0165-4608(86)90077-4.
A method was developed for obtaining direct chromosome preparations from SENCAR mouse skin tumors induced by chemical carcinogenesis protocols. Papillomas and squamous cell carcinomas were mechanically dispersed immediately after resection and were placed in a modified Hanks' solution with collagenase, trypsin, hyaluronidase, bovine albumin, and Colcemid. Total exposure to Colcemid did not exceed 1 hr. Metaphases were obtained in 100% of the analyzed specimens, allowing chromosome counting screening for double minutes and, in 50% of the cases, useful G-banded slides. The technique described has produced, for this type of tumor, a higher number of successful G-banded preparations than other previously reported methods for solid tumors. This procedure may be applicable for the study of human solid tumors that are histologically similar to our murine model, such as squamous cell carcinoma of cervix or lung.
开发了一种从化学致癌方案诱导的SENCAR小鼠皮肤肿瘤中获得直接染色体标本的方法。乳头状瘤和鳞状细胞癌在切除后立即进行机械分散，并置于含有胶原酶、胰蛋白酶、透明质酸酶、牛白蛋白和秋水仙酰胺的改良汉克斯溶液中。秋水仙酰胺的总暴露时间不超过1小时。在100%的分析标本中获得了中期染色体，可进行染色体计数、双微体筛查，并且在50%的病例中获得了有用的G带玻片。对于这种类型的肿瘤，所描述的技术比其他先前报道的实体瘤方法产生了更多成功的G带标本。该程序可能适用于组织学上与我们的小鼠模型相似的人类实体瘤研究，如宫颈癌或肺癌的鳞状细胞癌。
