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木霉菌株对四种鹰嘴豆致病镰刀菌分离株拮抗作用的体外和体内研究

In vitro and in vivo study of the antagonistic effects of a Trichoderma strain against four isolates of Fusarium that are pathogenic to chickpea.

作者信息

Adnani Manal, El Hazzat Naila, El Alaoui Moulay Abdelaziz, Selmaoui Karima, Benkirane Rachid, Ouazzani Touhami Amina, Douira Allal

机构信息

Laboratoire des Productions Végétales, Animales et Agro-industrie, Equipe de botanique, Biotechnologie et Protection des Plantes, Département de Biologie, Faculté des Sciences, Université Ibn Tofail, Campus Universitaire, BP 242 Kenitra, Morocco.

出版信息

3 Biotech. 2024 Nov;14(11):271. doi: 10.1007/s13205-024-04112-5. Epub 2024 Oct 18.

DOI:10.1007/s13205-024-04112-5
PMID:39430773
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11489381/
Abstract

This study investigated the antagonistic activity of against chickpea wilt through in vitro and in vivo experiments. The dual culture test showed that had a significant inhibitory effect on the growth of the tested isolates, with an inhibition rate ranging from 71.33% to 80.66%. The volatile and non-volatile metabolites produced by also showed antagonistic effects, with a growth inhibition rate ranging from 47.33% to 51.33% and a colonization rate ranging from 60% to 67%. In vivo experiments demonstrated that treating chickpea seeds with 48 h after inoculation with Fusarium significantly enhanced chickpea growth compared to seeds inoculated with alone. Arial part length enhancement ranged between 69.3% and 92,19% while root length increased by 61,9% and 127%, this implied a significant improvement in biomass. These findings highlight 's potential in controlling chickpea wilt and enhancing plant growth, making it an environmentally friendly method in sustainable agriculture and crop protection.

摘要

本研究通过体外和体内实验研究了[具体物质]对鹰嘴豆枯萎病的拮抗活性。双培养试验表明,[具体物质]对受试[病原菌名称]分离株的生长具有显著抑制作用,抑制率在71.33%至80.66%之间。[具体物质]产生的挥发性和非挥发性代谢产物也表现出拮抗作用,生长抑制率在47.33%至51.33%之间,定殖率在60%至67%之间。体内实验表明,与仅接种[病原菌名称]的种子相比,在接种镰刀菌48小时后用[具体物质]处理鹰嘴豆种子可显著促进鹰嘴豆生长。地上部分长度增加幅度在69.3%至92.19%之间,而根长增加了61.9%至127%,这意味着生物量有显著提高。这些发现凸显了[具体物质]在控制鹰嘴豆枯萎病和促进植物生长方面的潜力,使其成为可持续农业和作物保护中一种环保的方法。

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