In vitro and in vivo study of the antagonistic effects of a Trichoderma strain against four isolates of Fusarium that are pathogenic to chickpea.

This study investigated the antagonistic activity of against chickpea wilt through in vitro and in vivo experiments. The dual culture test showed that had a significant inhibitory effect on the growth of the tested isolates, with an inhibition rate ranging from 71.33% to 80.66%. The volatile and non-volatile metabolites produced by also showed antagonistic effects, with a growth inhibition rate ranging from 47.33% to 51.33% and a colonization rate ranging from 60% to 67%. In vivo experiments demonstrated that treating chickpea seeds with 48 h after inoculation with Fusarium significantly enhanced chickpea growth compared to seeds inoculated with alone. Arial part length enhancement ranged between 69.3% and 92,19% while root length increased by 61,9% and 127%, this implied a significant improvement in biomass. These findings highlight 's potential in controlling chickpea wilt and enhancing plant growth, making it an environmentally friendly method in sustainable agriculture and crop protection.