Rowe J M, Kasper S, Shiu R P, Friesen H G
Cancer Res. 1986 Mar;46(3):1408-12.
A growth factor has been purified to homogeneity from human mammary tumors. The human mammary tumor-derived growth factor (h.MTGF) has a molecular weight of 16,000 and an isoelectric point of 8.0 and is sensitive to heat, trypsin digestion, acid, and reduction. h.MTGF was purified to homogeneity using carboxymethylcellulose 52, heparin-Sepharose, and copper-Sepharose affinity chromatography. The stimulation of proliferation in cultured rabbit fetal chondrocytes was used as the principal bioassay. Purified h.MTGF was also mitogenic for bovine corneal endothelial cells, human fibroblasts, and a human breast cancer cell line, T-47D. It is postulated that h.MTGF may play a role in the fibrovascular changes of malignant breast tumors and promote the autonomous growth of the neoplastic cell population.
一种生长因子已从人乳腺肿瘤中纯化至同质。人乳腺肿瘤衍生生长因子（h.MTGF）分子量为16,000，等电点为8.0，对热、胰蛋白酶消化、酸和还原敏感。使用羧甲基纤维素52、肝素 - 琼脂糖和铜 - 琼脂糖亲和色谱法将h.MTGF纯化至同质。培养的兔胎儿软骨细胞增殖的刺激用作主要生物测定法。纯化的h.MTGF对牛角膜内皮细胞、人成纤维细胞和人乳腺癌细胞系T - 47D也有促有丝分裂作用。据推测，h.MTGF可能在恶性乳腺肿瘤的纤维血管变化中起作用，并促进肿瘤细胞群体的自主生长。
