Moeller J, Hültner L, Schmitt E, Breuer M, Dörmer P
Institut für Experimentelle Hämatologie, Gesellschaft für Strahlen und Umweltforschung, Munich, FRG.
J Immunol. 1990 Jun 1;144(11):4231-4.
A novel growth factor for bone marrow derived murine mucosal type mast cells has been isolated from the conditioned medium of the Mlsa-reactive mouse Th cell line MLS-4.2. In proliferation assays this growth factor synergizes, like IL-4, with IL-3 on established mast cell lines and was therefore termed MEA: mast cell growth enhancing activity. MEA was characterized as a glycoprotein with a Mr range between 37,000 and 43,000. Apparent homogeneity was obtained by using a four-step purification scheme including cation exchange chromatography, Procion red affinity chromatography, IEF, and gel filtration. Inasmuch as MEA was N-terminally blocked during automated Edman-degradation, peptide fragments after digestion with trypsin were used for partial amino acid sequence determination. All evaluable MEA peptide fragments showed complete sequence homology to a recently purified and cloned novel T cell growth factor (P40/TCGF III), the mouse homologue of human IL-9.
一种用于骨髓来源的小鼠黏膜型肥大细胞的新型生长因子已从Mlsa反应性小鼠T细胞系MLS-4.2的条件培养基中分离出来。在增殖试验中，这种生长因子与IL-4一样，在已建立的肥大细胞系上与IL-3协同作用，因此被称为MEA：肥大细胞生长增强活性。MEA被鉴定为一种糖蛋白，其分子量范围在37000至43000之间。通过包括阳离子交换色谱、Procion红亲和色谱、IEF和凝胶过滤在内的四步纯化方案获得了明显的均一性。由于MEA在自动Edman降解过程中N端被封闭，用胰蛋白酶消化后的肽片段用于部分氨基酸序列测定。所有可评估的MEA肽片段与最近纯化和克隆的新型T细胞生长因子（P40/TCGF III），即人IL-9的小鼠同源物，显示出完全的序列同源性。
