Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong, China.
Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong, China.
Physiol Plant. 2024 Sep-Oct;176(5):e14584. doi: 10.1111/ppl.14584.
Autophagy is a highly conserved method of recycling cytoplasm components in eukaryotes. It plays an important role in plant growth and development, as well as in response to biotic and abiotic stresses. Although autophagy-related genes (ATGs) have been identified in several crop species, their particular role in potato (Solanum tuberosum L.) remains unclear. Several transcription factors and signaling genes in the transgenic lines of the model plant Arabidopsis thaliana, such as AtTSPO, AtBES1, AtPIP2;7, AtCOST1 as well as AtATI1/2, ATG8f, GFP-ATG8F-HA, AtDSK2, AtNBR1, AtHKT1 play crucial functions under drought and salt stresses, respectively. In this study, a total of 29 putative StATGs from 15 different ATG subfamilies in the potato genome were identified. Their physicochemical properties, evolutionary connections, chromosomal distribution, gene duplication, protein-protein interaction network, conserved motifs, gene structure, interspecific collinearity relationship, and cis-regulatory elements were analyzed. The results of qRT-PCR detection of StATG expression showed that 29 StATGs were differentially expressed in potato's leaves, flowers, petiole, stem, stolon, tuber, and root. StATGs were dynamically modulated by salt and drought stresses and up-regulated under salt and drought conditions. Our results showed that the StATG8a localized in the cytoplasm and the nucleus. Potato cultivar "Atlantic" overexpressing or downregulating StATG8a were constructed. Based on physiological, biochemical, and photosynthesis parameters, potato lines overexpressing StATG8a exhibited 9 times higher drought and salt tolerance compared to non-transgenic plants. In contrast, the potato plants with knockdown expression showed a downtrend in drought and salt tolerance compared to non-transgenic potato lines. These results could provide new insights into the function of StATG8a in salt and drought response and its possible mechanisms.
自噬是真核生物中回收细胞质成分的一种高度保守的方法。它在植物的生长和发育中以及在应对生物和非生物胁迫中起着重要作用。尽管已经在几种作物物种中鉴定出了与自噬相关的基因（ATGs），但其在马铃薯（Solanum tuberosum L.）中的特定作用尚不清楚。拟南芥模式植物的转基因系中的几种转录因子和信号基因，如 AtTSPO、AtBES1、AtPIP2；7、AtCOST1 以及 AtATI1/2、ATG8f、GFP-ATG8F-HA、AtDSK2、AtNBR1、AtHKT1，在干旱和盐胁迫下分别发挥着至关重要的作用。在这项研究中，从马铃薯基因组中的 15 个不同的 ATG 亚家族中共鉴定出了 29 个推定的 StATGs。分析了它们的理化性质、进化关系、染色体分布、基因复制、蛋白质-蛋白质相互作用网络、保守基序、基因结构、种间共线性关系和顺式调控元件。StATG 表达的 qRT-PCR 检测结果表明，29 个 StATGs 在马铃薯的叶片、花朵、叶柄、茎、匍匐茎、块茎和根中差异表达。StATGs 受盐和干旱胁迫的动态调节，并在盐和干旱条件下上调。我们的结果表明，StATG8a 定位于细胞质和细胞核中。构建了马铃薯品种“Atlantic”过表达或下调 StATG8a 的植株。基于生理、生化和光合作用参数，过表达 StATG8a 的马铃薯品系表现出比非转基因植物高 9 倍的耐旱性和耐盐性。相比之下，具有敲低表达的马铃薯植物在耐旱性和耐盐性方面与非转基因马铃薯品系相比呈下降趋势。这些结果可以为 StATG8a 在盐和干旱响应中的功能及其可能的机制提供新的见解。
