Achari R, Drissel D, Hulse J D
Clin Chem. 1986 Feb;32(2):374-6.
We describe a simple, reproducible liquid-chromatographic method for determination of esmolol (a short-acting beta blocker) and its major metabolite in human urine. Esmolol is extracted from urine at a pH of 8.4 into methylene chloride; the more polar metabolite remains in the aqueous phase. We then measure esmolol with a muBondapak C18 column and measure ultraviolet absorbance at 229 nm; the metabolite is analyzed with a Spherisorb phenyl column, with absorbance measured at 280 nm. The average extraction recoveries of esmolol and the metabolite were 95 and 92%, respectively. Standard curves were linear and reproducible for esmolol from 0.025 to 5 mg/L and for the metabolite from 1 to 250 mg/L. Within-day CVs for both compounds were less than 6%.
我们描述了一种简单、可重复的液相色谱法，用于测定人尿中艾司洛尔（一种短效β受体阻滞剂）及其主要代谢物。艾司洛尔在pH值为8.4的条件下从尿液中萃取到二氯甲烷中；极性更强的代谢物则留在水相中。然后，我们使用μBondapak C18柱测定艾司洛尔，并在229 nm处测量紫外吸光度；使用Spherisorb苯基柱分析代谢物，在280 nm处测量吸光度。艾司洛尔和代谢物的平均萃取回收率分别为95%和92%。艾司洛尔浓度在0.025至5 mg/L范围内以及代谢物浓度在1至250 mg/L范围内时，标准曲线呈线性且可重复。两种化合物的日内变异系数均小于6%。
