Duncan Robin, Mantegazza Giacomo, Gargari Giorgio, Pierallini Elena, Russo Rosario, Guglielmetti Simone
Division of Food Microbiology and Bioprocesses, Department of Food, Environment, and Nutritional Science, Università Degli Studi Di Milano, Via Celoria 2, 20133, Milan, Italy.
μbEat Lab, Department of Biotechnology and Biosciences (BtBs), University of Milano-Bicocca, Piazza Della Scienza 4, 20133, Milan, Italy.
Probiotics Antimicrob Proteins. 2024 Oct 21. doi: 10.1007/s12602-024-10383-4.
Ensuring the viability and efficacy of probiotic microorganisms during manufacturing and gastrointestinal transit remains challenging, particularly for sensitive strains such as certain lactic acid bacteria and bifidobacteria. This has led to increased interest in spore-forming bacteria, such as Heyndrickxia coagulans (formerly Bacillus coagulans), which can endure environmental stresses through their endospore forms. This study presents a comprehensive analysis of the probiotic potential of strain LMG S-24828, originally isolated from healthy human feces. The genomic analysis confirmed the strain's taxonomic placement within the species H. coagulans and revealed no extrachromosomal plasmid DNA, suggesting genetic stability. Safety assessments demonstrated that LMG S-24828 does not produce D-lactate, deconjugate bile salts, or exhibit hemolytic activity, and it lacks transmissible antibiotic resistances. Phenotypic tests showed the strain's metabolic versatility, including its ability to hydrolyze complex carbohydrates and adhere to intestinal epithelial cells. Moreover, LMG S-24828 exhibited robust survival and germination during in vitro and in vivo gastrointestinal simulations, with evidence of significant spore germination in the human gut. These findings suggest that H. coagulans LMG S-24828 possesses several advantageous traits for probiotic applications, warranting further clinical evaluation to confirm its health benefits.
在生产过程和胃肠道转运过程中确保益生菌微生物的活力和功效仍然具有挑战性，对于某些乳酸菌和双歧杆菌等敏感菌株而言尤其如此。这使得人们对产芽孢细菌的兴趣增加，例如凝结海因德里克氏菌（以前称为凝结芽孢杆菌），它们可以通过其内生孢子形式承受环境压力。本研究对最初从健康人类粪便中分离出的LMG S-24828菌株的益生菌潜力进行了全面分析。基因组分析证实了该菌株在凝结海因德里克氏菌物种中的分类地位，并揭示没有染色体外质粒DNA，表明其遗传稳定性。安全性评估表明，LMG S-24828不产生D-乳酸、不使结合胆汁盐解偶联或表现出溶血活性，并且它缺乏可传播的抗生素抗性。表型测试显示该菌株具有代谢多样性，包括其水解复杂碳水化合物和粘附于肠上皮细胞的能力。此外，LMG S-24828在体外和体内胃肠道模拟过程中表现出强大的存活和萌发能力，有证据表明其在人体肠道中能显著萌发。这些发现表明，凝结海因德里克氏菌LMG S-24828具有若干益生菌应用的有利特性，值得进一步进行临床评估以确认其对健康的益处。
