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来自亚洲旱地的沙漠蝎子——东亚钳蝎的染色体水平基因组组装。

A chromosome-level genome assembly for the desert scorpion Mesobuthus przewalskii from Asian drylands.

作者信息

Su Yan-Kai, Xiu Ming-Hua, Yang Hao-Yan, Shi Cheng-Min

机构信息

State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding 071001, China.

Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.

出版信息

J Hered. 2024 Oct 21. doi: 10.1093/jhered/esae059.

Abstract

Scorpions are an ancient and charismatic group of arthropods with medical importance, but a high-quality reference genome for this group is still lacking. Here we perform whole-genome sequencing of Mesobuthus przewalskii, a desert scorpion endemic to the Taklimakan Desert. We combine PacBio HiFi sequencing and Hi-C chromosome conformation capturing to generate chromosomal-level, haplotype-resolved, and fully annotated genome assembly for this medically important scorpion. The assembly consists of two haplotypes (1052.01 Mbp and 1055.19 Mbp, respectively) reaching chromosome-level contiguity and >98% BUSCO completeness. Sequences were anchored in 13 chromosomes with a contig N50 of 34.44 Mbp and scaffold N50 of 81.43 Mbp. Several key genome features and the mitochondrial genome assembly were also provided. This genome represents the fifth but the most complete assembly for the order Scorpiones.

摘要

蝎子是一类古老且具有魅力的节肢动物,具有医学重要性,但该类群仍缺乏高质量的参考基因组。在此,我们对一种分布于塔克拉玛干沙漠的特有沙漠蝎——西藏琵蝎(Mesobuthus przewalskii)进行了全基因组测序。我们结合了PacBio HiFi测序和Hi-C染色体构象捕获技术,为这种具有医学重要性的蝎子生成了染色体水平、单倍型解析且注释完整的基因组组装。该组装由两个单倍型(分别为1052.01 Mbp和1055.19 Mbp)组成,达到了染色体水平的连续性,且BUSCO完整性大于98%。序列被锚定在13条染色体上,重叠群N50为34.44 Mbp,支架N50为81.43 Mbp。还提供了几个关键的基因组特征和线粒体基因组组装。该基因组是蝎目动物的第五个但也是最完整的组装。

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