Feng Xiu, Zhu Ren, Jia Yintao, Sui Xiaoyun, Wang Xiaoling, Zhuo Yu, Li Junle, Chen Yifeng
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Sci Data. 2025 Jul 1;12(1):1082. doi: 10.1038/s41597-025-05122-5.
Triplophysa scleroptera is an endemic fish species in Qinghai Lake and the upper reaches of the Yellow River. However, studies on conservation and evolutionary genetics were seriously impeded by the absence of a reference genome. Here, by using PacBio HiFi sequencing and Hi-C assembly technology, we assembled a chromosome-level genome of T. scleroptera, with a total length of 660.22 Mb and 99.82% of the sequence anchored to 25 chromosomes. The contig N50 and scaffold N50 were 9.09 Mb and 24.38 Mb, respectively. The evaluation using BUSCO indicated the genome assembly to be 96.40% complete. About 33.41% of the genome consists of repeat elements. We predicted 26,168 protein-coding genes in the genome, and 99.02% of them were functionally annotated. This high-quality reference genome would serve as a valuable genomic resource for advancing evolutionary conservation genetics studies in this species.
硬鳍高原鳅是青海湖和黄河上游的特有鱼类物种。然而，由于缺乏参考基因组，其保护和进化遗传学研究受到严重阻碍。在此，我们利用PacBio HiFi测序和Hi-C组装技术，组装了硬鳍高原鳅的染色体水平基因组，全长660.22 Mb，99.82%的序列锚定到25条染色体上。重叠群N50和支架N50分别为9.09 Mb和24.38 Mb。使用BUSCO评估表明基因组组装完整性为96.40%。基因组约33.41%由重复元件组成。我们在基因组中预测了26168个蛋白质编码基因，其中99.02%得到了功能注释。这个高质量的参考基因组将为推进该物种的进化保护遗传学研究提供有价值的基因组资源。
