Maoka T, Arai A, Shimizu M, Matsuno T
Comp Biochem Physiol B. 1986;83(1):121-4. doi: 10.1016/0305-0491(86)90341-x.
Racemic mixtures of (3RS, 3'RS)-zeaxanthin were separated into the three optical isomers, (3R, 3'R)-zeaxanthin (1), (3R,3'S;meso)-zeaxanthin (2) and (3S,3'S)-zeaxanthin (3), by converting to their corresponding dibenzoates and by using HPLC on an optical resolution column Sumipax OA-2000. According to this procedure, it has been shown that only (1) is isolated from higher plants, shellfish, starfish, sea squirt, sea cucumber and then examined; on the other hand (1), (2) and (3) are isolated from zeaxanthin fraction of shrimp, fish and turtle examined. This is the first isolation of enantiomeric and meso-zeaxanthin in nature.
通过将（3RS，3'RS）-玉米黄质的外消旋混合物转化为其相应的二苯甲酸酯，并使用手性拆分柱Sumipax OA-2000进行高效液相色谱法，将其分离为三种旋光异构体，即（3R，3'R）-玉米黄质（1）、（3R，3'S；内消旋）-玉米黄质（2）和（3S，3'S）-玉米黄质（3）。根据此方法，已表明仅从高等植物、贝类、海星、海鞘、海参中分离出（1）并进行了检测；另一方面，从所检测的虾、鱼和龟的玉米黄质组分中分离出了（1）、（2）和（3）。这是首次在自然界中分离出对映体和内消旋玉米黄质。
