海七鳃鳗（Petromyzon marinus L.）生命周期中血清蛋白的电泳和免疫电泳研究

An electrophoretic and immunoelectrophoretic study of serum proteins during the life cycle of the lamprey, Petromyzon marinus L.

作者信息

Filosa M F, Sargent P A, Youson J H

出版信息

Comp Biochem Physiol B. 1986;83(1):143-9. doi: 10.1016/0305-0491(86)90344-5.

DOI:10.1016/0305-0491(86)90344-5

Abstract

Serum proteins of upstream migrants, parasitic adults, early and late metamorphosing animals, and ammocoetes of Petromyzon marinus L. were examined by polyacrylamide gel electrophoresis and crossed-immunoelectrophoresis. The electrophoretic patterns for two proteins, SDS-1 and CB-III, were found to change during the life cycle. quantitative measurements of these two proteins showed that they continued to increase until in the adult they together constituted approx. 85% of the total serum protein. Evidence was obtained for a protein present in ammocoetes and metamorphosing animals, but not in upstream migrants.

摘要

通过聚丙烯酰胺凝胶电泳和交叉免疫电泳对海七鳃鳗（Petromyzon marinus L.）溯河洄游个体、寄生成虫、变态发育早期和晚期个体以及沙隐虫的血清蛋白进行了检测。发现两种蛋白SDS - 1和CB - III的电泳图谱在生命周期中发生变化。对这两种蛋白的定量测量表明，它们持续增加，直至在成体中它们共同构成了约85%的血清总蛋白。有证据表明，一种蛋白存在于沙隐虫和变态发育个体中，但在溯河洄游个体中不存在。

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