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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Filosa M F, Sargent P A, Fisher M M, Youson J H

Comp Biochem Physiol B. 1982;72(4):521-30. doi: 10.1016/0305-0491(82)90500-4.

Serum proteins of upstream migrants of Petromyzon marinus were separated electrophoretically on polyacrylamide and agarose as well as by crossed immunoelectrophoresis using anti-lamprey serum antiserum. 2. Some constituents of four of the five major bands separated by electrophoresis on polyacrylamide were located in the agarose and crossed-immunoelectrophoretic patterns. 3. Two serum proteins, SDS-1 and CB-III, were isolated. SDS-1 was found to be a glycoprotein; CB-III may be a lipoprotein. 4. Monospecific antisera were produced against SDS-1 and CB-III and were used in crossed immunoelectrophoresis to analyze lamprey serum. This method revealed that electrophoretically different forms of these two proteins exist in serum. 5. Immunoelectrophoretic methods demonstrated that three of the major bands produced by polyacrylamide gel electrophoresis of whole serum contained more than a single protein.

利用抗七鳃鳗血清抗血清，通过聚丙烯酰胺和琼脂糖凝胶电泳以及交叉免疫电泳，对海七鳃鳗溯河洄游群体的血清蛋白进行了分离。2. 在聚丙烯酰胺凝胶电泳中分离出的五条主要条带中的四条的某些成分，在琼脂糖凝胶电泳和交叉免疫电泳图谱中也有显示。3. 分离出了两种血清蛋白，即SDS - 1和CB - III。发现SDS - 1是一种糖蛋白；CB - III可能是一种脂蛋白。4. 制备了针对SDS - 1和CB - III的单特异性抗血清，并用于交叉免疫电泳分析七鳃鳗血清。该方法表明血清中存在这两种蛋白质的电泳不同形式。5. 免疫电泳方法表明，全血清聚丙烯酰胺凝胶电泳产生的三条主要条带中每条都包含不止一种蛋白质。

Filosa M F, Sargent P A, Fisher M M, Youson J H

Comp Biochem Physiol B. 1982;72(4):521-30. doi: 10.1016/0305-0491(82)90500-4.

Serum proteins of upstream migrants of Petromyzon marinus were separated electrophoretically on polyacrylamide and agarose as well as by crossed immunoelectrophoresis using anti-lamprey serum antiserum. 2. Some constituents of four of the five major bands separated by electrophoresis on polyacrylamide were located in the agarose and crossed-immunoelectrophoretic patterns. 3. Two serum proteins, SDS-1 and CB-III, were isolated. SDS-1 was found to be a glycoprotein; CB-III may be a lipoprotein. 4. Monospecific antisera were produced against SDS-1 and CB-III and were used in crossed immunoelectrophoresis to analyze lamprey serum. This method revealed that electrophoretically different forms of these two proteins exist in serum. 5. Immunoelectrophoretic methods demonstrated that three of the major bands produced by polyacrylamide gel electrophoresis of whole serum contained more than a single protein.

利用抗七鳃鳗血清抗血清，通过聚丙烯酰胺和琼脂糖凝胶电泳以及交叉免疫电泳，对海七鳃鳗溯河洄游群体的血清蛋白进行了分离。2. 在聚丙烯酰胺凝胶电泳中分离出的五条主要条带中的四条的某些成分，在琼脂糖凝胶电泳和交叉免疫电泳图谱中也有显示。3. 分离出了两种血清蛋白，即SDS - 1和CB - III。发现SDS - 1是一种糖蛋白；CB - III可能是一种脂蛋白。4. 制备了针对SDS - 1和CB - III的单特异性抗血清，并用于交叉免疫电泳分析七鳃鳗血清。该方法表明血清中存在这两种蛋白质的电泳不同形式。5. 免疫电泳方法表明，全血清聚丙烯酰胺凝胶电泳产生的三条主要条带中每条都包含不止一种蛋白质。