College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, PR China.
Gansu Gannan Animal Husbandry and Veterinary Workstation, Gannan 747000, PR China.
Ultrason Sonochem. 2024 Dec;111:107102. doi: 10.1016/j.ultsonch.2024.107102. Epub 2024 Oct 16.
In this paper, a new type of antifreeze peptide was identified from the ultrasonic-assisted enzymolysis product of yak skin. First, the antifreeze peptides were obtained by ultrasonic-assisted enzymolysis of yak skin gelatin. The obtained peptides with 150 W ultrasound treatment could increase the survival rate of Lactobacillus plantarum from 16 % to 60 % after four freeze-thaw cycles. Then, the component with the highest antifreeze activity in the peptides' ultrafiltration product was analyzed by LC-MS/MS to obtain 961 peptides. After screening of antifreeze activity and physical properties, three antifreeze peptide sequences were obtained (S1: GERGGPGGPGPQ, S2: PGGAEGPGRDAQQP, S3: VAPPGAPKKEH). DSC analysis, Lactobacillus plantarum cryoprotection experiments and molecular docking showed that the S1 sequence had the best antifreeze activity. This study provides a new idea for the high-value utilization of yak by-products and a potential candidate for food antifreeze agents.
本文从牦牛皮超声辅助酶解产物中鉴定出一种新型抗冻肽。首先，通过牦牛皮明胶的超声辅助酶解获得抗冻肽。用 150 W 的超声处理获得的肽，在经过四轮冻融循环后，可将植物乳杆菌的存活率从 16%提高到 60%。然后，通过 LC-MS/MS 对肽超滤产物中具有最高抗冻活性的成分进行分析，得到 961 条肽。经过抗冻活性和物理性质的筛选，得到了三个抗冻肽序列（S1：GERGGPGGPGPQ、S2：PGGAEGPGRDAQQP、S3：VAPPGAPKKEH）。DSC 分析、植物乳杆菌保护实验和分子对接表明，S1 序列具有最佳的抗冻活性。本研究为牦牛副产物的高值化利用提供了新的思路，为食品抗冻剂提供了潜在的候选物。
