Kibar Mustafa, Aytekin İbrahim
Faculty of Agriculture, Department of Animal Science, University of Siirt, 56100 Siirt, Türkiye.
Faculty of Agriculture, Department of Animal Science, University of Selçuk, 42130 Konya, Türkiye.
Gene. 2025 Mar 10;940:149027. doi: 10.1016/j.gene.2024.149027. Epub 2024 Oct 20.
Sustainability in dairy cattle farms depends on the efficiency of milk yield and reproductive traits. Thus, this study aimed to investigate the effect of the FGF-2/Csp6I gene and major environmental factors on these traits in Holstein-Friesian cattle. A total of 212 whole blood samples were collected from the Vena coccygea of cattle and the data obtained from these samples were used in all statistical analyses. Then, the restriction fragment length polymorphism (RFLP) method (determination of genotypes) was conducted and programs including PopGene (allele and genotype frequencies), Minitab (association analyses) and MTDFREML (variance components and genetic parameters) were used. Alleles A (0.4269) and G (0.5731) as well as genotypes AA (0.174), AG (0.505) and GG (0.321) were found, indicating that the population is polymorphic and in Hardy-Weinberg equilibrium (P > 0.05). The effect of the Csp6I polymorphism of FGF-2 gene on peak milk yield (PMY) (P < 0.01); lactation milk yield (LMY), milking time (MT), 305-day and 200-day lactation milk yield (LMY and LMY), average daily milk yield (ADMY) (P < 0.05); 100-day lactation milk yield (LMY), age of using in first breeding (AUFB) and number of inseminations per conception (NIPC) (P < 0.10) were significant. The heritability of milk yield traits and the correlation between direct and maternal heritability for reproductive traits were high. Furthermore, the breeding value of PMY was higher for the AA genotype (0.745 ± 0.292) than for the AG genotype (-0.268 ± 0.171) (P < 0.05). As a result, the A allele and AA genotype for the FGF-2/Csp6I gene had an increasing effect on milk yield without compromising reproductive performance in Holstein-Friesian dairy cattle.
奶牛场的可持续性取决于产奶量和繁殖性状的效率。因此，本研究旨在调查FGF - 2/Csp6I基因和主要环境因素对荷斯坦 - 弗里生奶牛这些性状的影响。从奶牛的尾静脉采集了总共212份全血样本，并将从这些样本中获得的数据用于所有统计分析。然后，进行了限制性片段长度多态性（RFLP）方法（基因型测定），并使用了包括PopGene（等位基因和基因型频率）、Minitab（关联分析）和MTDFREML（方差成分和遗传参数）在内的程序。发现等位基因A（0.4269）和G（0.5731）以及基因型AA（0.174）、AG（0.505）和GG（0.321），表明该群体具有多态性且处于哈迪 - 温伯格平衡（P > 0.05）。FGF - 2基因的Csp6I多态性对产奶高峰量（PMY）（P < 0.01）；泌乳期产奶量（LMY）、挤奶时间（MT）、305天和200天泌乳期产奶量（LMY和LMY）、平均日产奶量（ADMY）（P < 0.05）；100天泌乳期产奶量（LMY）、首次配种使用年龄（AUFB）和每次受孕输精次数（NIPC）（P < 0.10）的影响显著。产奶性状的遗传力以及繁殖性状的直接遗传力和母体遗传力之间的相关性较高。此外，AA基因型（0.745 ± 0.292）的PMY育种值高于AG基因型（-0.268 ± 0.171）（P < 0.05）。结果，FGF - 2/Csp6I基因的A等位基因和AA基因型对荷斯坦 - 弗里生奶牛的产奶量有增加作用，且不影响繁殖性能。
