Utomo Budi, Rimayanti Rimayanti, Kurnijasanti Rochmah, Degu Nurhusien Yimer, Diansyah Athhar Manabi, Amrullah Muhammad Fajar
Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, East Jawa, Indonesia.
Division of Basic Veterinary, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, East Jawa, Indonesia.
Vet World. 2025 Jul;18(7):1799-1806. doi: 10.14202/vetworld.2025.1799-1806. Epub 2025 Jul 8.
The () gene is recognized as a critical regulator of ovarian function and fertility in cattle. However, its role in crossbred populations, particularly Madrasin cattle (Madura × Simmental cross), remains underexplored. Understanding the genetic underpinnings of fertility traits in this crossbreed could provide valuable insights for improving reproductive efficiency in Indonesia's livestock sector. This study aimed to investigate the relationship between gene expression and reproductive traits, specifically reproductive tract size (RTS), cervical mucus characteristics, and fertility rate, in Madrasin cows.
A total of 20 Madrasin cows (aged 2-5 years) were evaluated. expression levels were assessed through polymerase chain reaction, and samples were categorized into "Strong Expression" (G1) and "Dimmed Expression" (G2) groups based on amplicon band intensity. Reproductive tract dimensions were recorded through rectal palpation, while cervical mucus quality was analyzed using five parameters: appearance, viscosity, spinnbarkeit, pH, and fern pattern. Fertility was determined by the non-return rate (NRR) following artificial insemination. Statistical analysis was conducted using analysis of variance with a significance threshold of p < 0.05.
Cows in the strong expression group exhibited significantly larger uterine horn diameters and greater ovarian height than the dimmed group (p < 0.05). Cervical mucus from G1 animals displayed higher scores in appearance and spinnbarkeit, although only appearance was statistically significant. NRR was notably higher in G1 (p < 0.05), suggesting enhanced fertility in cows with elevated expression.
gene expression in Madrasin cattle is positively associated with RTS and fertility-related cervical mucus characteristics. These findings suggest that could serve as a promising genetic marker for improving fertility and breeding outcomes in crossbred cattle populations. However, the study's limited sample size and exclusion of environmental factors warrant further investigation to validate the utility of in broader genetic selection strategies.
（）基因被认为是牛卵巢功能和生育能力的关键调节因子。然而，其在杂交群体，特别是马德拉斯牛（马都拉牛×西门塔尔牛杂交种）中的作用仍未得到充分研究。了解该杂交品种生育性状的遗传基础可为提高印度尼西亚畜牧业的繁殖效率提供有价值的见解。本研究旨在调查马德拉斯牛中（）基因表达与繁殖性状之间的关系，特别是生殖道大小（RTS）、宫颈黏液特征和生育率。
共评估了20头年龄在2至5岁的马德拉斯牛。通过聚合酶链反应评估（）基因表达水平，并根据扩增带强度将样本分为“强表达”（G1）和“弱表达”（G2）组。通过直肠触诊记录生殖道尺寸，同时使用外观、粘度、延展性、pH值和蕨类图案五个参数分析宫颈黏液质量。通过人工授精后的返情率（NRR）确定生育能力。使用方差分析进行统计分析，显著性阈值为p < 0.05。
强（）基因表达组的母牛子宫角直径明显大于弱表达组，卵巢高度也更高（p < 0.05）。G1组动物的宫颈黏液在外观和延展性方面得分更高，尽管只有外观具有统计学意义。G1组的NRR显著更高（p <
