Department of Microbiology, Department of Internal Medicine, Al-Nahrain University, Baghdad, Iraq.
Department of Internal Medicine, Al-Nahrain University, Baghdad, Iraq.
J Pak Med Assoc. 2024 Oct;74(10 (Supple-8)):S181-S185. doi: 10.47391/JPMA-BAGH-16-40.
To evaluate the ratio of interleukin18 and interleukin18 binding protein in type 2 diabetes mellitus patients, and its relation with the presence of interleukin18-607C/A and interleukin18-137G/C gene polymorphisms and the type of complications.
The case-control study was conducted at the endocrinology clinic of the Madinat Al-Imamin Al-Kazemin Teaching Hospital, Iraq, from September 2020 to July 2021, and comprised diagnosed patients of type 2 diabetes mellitus, and healthy controls matched for age and gender. Blood samples were obtained that were processed for serum separation. Serum interleukin18 and interleukin18 binding protein levels were assessed, and part of the sample was used for deoxyribonucleic acid extraction using tetra-primer amplification refractory mutation system polymerase chain reaction with four specific primers for interleukin18-607C/A and interleukin-18-137G/C gene polymorphisms in both the groups. Data was analysed using SPSS 20.
Of the 168 subjects, 86(51.2%) were patients; 67(77.9%) females and 19(22.1%) males with mean age 52±8.97 years. There were 82(48.8%) controls; 56(68.3%) females and 26(31.7%) males with mean age 48±9.44 years (p>0.05). Higher serum level of interleukin18 and lower level of interleukin18 binding protein were seen in type 2 diabetes mellitus group compared to the controls (p<0.001). Interleukin18-137G/C and interleukin18-607C/A mutant alleles had odd ratios 3.52 (confidence interval: 1.91- 6.63) and 3.25 (confidence interval: 1.77-5.83), respectively, as risk factors for the occurrence of type 2 diabetes mellitus. There was an association of interleukin18- 137G/C homozygous mutant genotype with the occurrence of retinopathy among type 2 diabetes mellitus patients (p=0.046), but risk allele C was not associated with retinopathy (p>0.05).
The presence of interleukin18-137G/C and interleukin18-607C/A gene polymorphism might be considered a risk factor for type 2 diabetes mellitus.
评估 2 型糖尿病患者白细胞介素 18（IL-18）与白细胞介素 18 结合蛋白的比值及其与白细胞介素 18-607C/A 和白细胞介素 18-137G/C 基因多态性的存在以及并发症类型的关系。
该病例对照研究于 2020 年 9 月至 2021 年 7 月在伊拉克 Madinat Al-Imamin Al-Kazemin 教学医院的内分泌科进行，纳入了确诊为 2 型糖尿病的患者和年龄与性别相匹配的健康对照组。采集血液样本，分离血清。评估血清白细胞介素 18 和白细胞介素 18 结合蛋白水平，并使用四引物扩增受阻突变系统聚合酶链反应（polymerase chain reaction，PCR）从部分样本中提取脱氧核糖核酸（deoxyribonucleic acid，DNA），以检测两组白细胞介素 18-607C/A 和白细胞介素-18-137G/C 基因多态性的四个特定引物。使用 SPSS 20 对数据进行分析。
在 168 名受试者中，86 名（51.2%）为患者；女性 67 名（77.9%），男性 19 名（22.1%），平均年龄 52±8.97 岁。对照组有 82 名（48.8%）；女性 56 名（68.3%），男性 26 名（31.7%），平均年龄 48±9.44 岁（p>0.05）。与对照组相比，2 型糖尿病组血清白细胞介素 18 水平较高，白细胞介素 18 结合蛋白水平较低（p<0.001）。白细胞介素 18-137G/C 和白细胞介素 18-607C/A 突变等位基因的优势比（odds ratio，OR）分别为 3.52（95%置信区间：1.91-6.63）和 3.25（95%置信区间：1.77-5.83），是 2 型糖尿病的发生危险因素。白细胞介素 18-137G/C 纯合突变基因型与 2 型糖尿病患者视网膜病变的发生有关（p=0.046），但风险等位基因 C 与视网膜病变无关（p>0.05）。
白细胞介素 18-137G/C 和白细胞介素 18-607C/A 基因多态性的存在可能被认为是 2 型糖尿病的危险因素。
