Erexson G L, Wilmer J L, Steinhagen W H, Kligerman A D
Environ Mutagen. 1986;8(1):29-40. doi: 10.1002/em.2860080104.
Experiments were designed to investigate both the induction of sister chromatid exchanges (SCEs) in peripheral blood lymphocytes (PBLs) and micronuclei (MN) in bone marrow polychromatic erythrocytes (PCEs) of mice and rats after inhalation of benzene (BZ). Male DBA/2 mice (17-19 weeks old) were exposed to target concentrations of either 0, 10, 100, or 1,000 ppm BZ for 6 hr. Male Sprague-Dawley rats (11-14 weeks old) were exposed to target concentrations of either 0, 0.1, 0.3, 1, 3, 10, or 30 ppm BZ for 6 hr. Blood was obtained by cardiac puncture 18 hr after exposure, and PBLs were cultured in the presence of lipopolysaccharide (mouse B cells, 60 micrograms/ml) or concanavalin A (rat T cells, 30 micrograms/ml) to stimulate blastogenesis for SCE analysis. Femoral bone marrow smears from both species were analyzed for MN in PCEs 18 hr after BZ exposure. Mouse PBLs revealed a significant concentration-related increase in the SCE frequency over controls at 10, 100, or 1,000 ppm BZ. Mouse bone marrow showed a significant concentration-dependent increase in MN over controls after exposure to 10, 100, or 1,000 ppm BZ. Rat PBLs showed a significant increase in the SCE frequency after exposure to 3, 10, or 30 ppm BZ. The statistical significance of the 1 ppm BZ result was borderline and dependent on the statistical test chosen. Rat cells revealed a significant concentration-related increase in MN after inhalation of either 1, 3, 10, or 30 ppm BZ. PBLs from treated mice showed significant concentration-dependent decreases in mitotic indices; however, cell cycle kinetics and leucocyte counts remained unaffected. Rat PBLs showed significant decreases in mitotic activity only after exposure to 3 and 30 ppm BZ, whereas cell cycle kinetics and leucocyte counts were unaffected. These results show that BZ can induce statistically significant cytogenetic effects in PBLs and PCEs of both mice and rats after a 6-hr inhalation of BZ at low concentrations.
实验旨在研究吸入苯（BZ）后，小鼠和大鼠外周血淋巴细胞（PBLs）中姐妹染色单体交换（SCEs）的诱导情况以及骨髓嗜多染红细胞（PCEs）中的微核（MN）情况。17 - 19周龄的雄性DBA/2小鼠暴露于浓度分别为0、10、100或1000 ppm的目标BZ浓度下6小时。11 - 14周龄的雄性Sprague - Dawley大鼠暴露于浓度分别为0、0.1、0.3、1、3、10或30 ppm的目标BZ浓度下6小时。暴露18小时后通过心脏穿刺取血，将PBLs在脂多糖（小鼠B细胞，60微克/毫升）或伴刀豆球蛋白A（大鼠T细胞，30微克/毫升）存在的情况下培养以刺激细胞增殖用于SCE分析。两种动物在BZ暴露18小时后，对股骨骨髓涂片进行PCEs中MN的分析。小鼠PBLs在BZ浓度为10、100或1000 ppm时，SCE频率相对于对照组呈现出显著的浓度相关增加。小鼠骨髓在暴露于10、100或1000 ppm BZ后，MN相对于对照组呈现出显著的浓度依赖性增加。大鼠PBLs在暴露于3、10或30 ppm BZ后，SCE频率显著增加。1 ppm BZ结果的统计学显著性处于临界状态，并且取决于所选择的统计检验。大鼠细胞在吸入1、3、10或30 ppm BZ后，MN呈现出显著的浓度相关增加。经处理小鼠的PBLs有丝分裂指数呈现出显著的浓度依赖性降低；然而，细胞周期动力学和白细胞计数未受影响。大鼠PBLs仅在暴露于3和30 ppm BZ后有丝分裂活性显著降低，而细胞周期动力学和白细胞计数未受影响。这些结果表明，在低浓度下吸入BZ 6小时后，BZ可在小鼠和大鼠的PBLs和PCEs中诱导出具有统计学显著性的细胞遗传学效应。
