Kurata S, Tsukakoshi M, Kasuya T, Ikawa Y
Exp Cell Res. 1986 Feb;162(2):372-8. doi: 10.1016/0014-4827(86)90342-3.
A minute hole upon a cultured cell, perforated with a finely focused laser beam, was found to repair itself within a short period of time. The procedure constitutes a new way of introducing exogenous gene materials dissolved in medium into cells. The 'laser-aided' DNA transfection is better than the existing methods because it allows the treatment of a large number of cells in a shorter time, and an improved success rate.
人们发现，用聚焦精细的激光束在培养细胞上打出的微小孔洞能在短时间内自行修复。该过程构成了一种将溶解在培养基中的外源基因材料导入细胞的新方法。“激光辅助”DNA转染比现有方法更优，因为它能在更短时间内处理大量细胞，且成功率更高。
