杜氏丝状线虫：对小鼠高免疫反应性和低免疫反应性选择的直接和相关反应

Nematospiroides dubius: direct and correlated responses to selection for high and low immune responsiveness in mice.

作者信息

Sitepu P, Brindley P J, Dobson C

出版信息

Exp Parasitol. 1986 Feb;61(1):57-64. doi: 10.1016/0014-4894(86)90135-9.

Abstract

High and low immune responder lines of mice were bred selectively from an allogeneic stock over 10 generations, based on their fecal parasite egg count assayed 3 weeks after reinfection with 100 Nematospiroides dubius larvae. By generation 10, (F10), the low immune response mice voided about 10 times as many fecal N. dubius eggs as the high immune response mice. Realized heritability for the selected trait, fecal egg count after secondary infection (= protective immunity), was 0.35 at F7. F7 was considered the selection limit. Selection for change in fecal egg count did not significantly influence the conformational nor reproductive characteristics of these mice. Significant phenotypic and genetic correlations were evident between the selected character and innate immunity to N. dubius, humoral antibody response to N. dubius infection, and establishment, growth, and reproduction of N. dubius in the selected mice.

摘要

根据用100条杜氏线虫幼虫再次感染3周后测定的粪便寄生虫卵数，从一个同种异体种群中选择性培育出高免疫应答和低免疫应答品系的小鼠，历经10代。到第10代（F10）时，低免疫应答小鼠排出的粪便中杜氏线虫卵数量约为高免疫应答小鼠的10倍。在F7时，所选性状（二次感染后的粪便卵数，即保护性免疫力）的实现遗传力为0.35。F7被视为选择极限。对粪便卵数变化的选择并未显著影响这些小鼠的形态或繁殖特征。所选性状与对杜氏线虫的先天免疫力、对杜氏线虫感染的体液抗体反应以及杜氏线虫在所选小鼠体内的建立、生长和繁殖之间存在显著的表型和遗传相关性。