Hassell T M, Provenza D V, Foster R A
Experientia. 1986 Jan 15;42(1):66-9. doi: 10.1007/BF01975899.
The percentage of synthesis dedicated to collagen is elevated in low-density cultures of human gingival fibroblasts, as is per-cell total protein synthetic activity and glycosaminoglycan accumulation. These observations can be explained, in part, by a decrease in membrane transport of precursor substance in high-density cultures. Synthetic activity by human fibroblasts can be reliably assayed in vitro using as few as 500 cells sparsely seeded. Such low-cell number assay is essential for study of single-cell clones, where replicative life span is limited.
在人牙龈成纤维细胞的低密度培养物中，用于胶原蛋白合成的比例会升高，每细胞的总蛋白合成活性和糖胺聚糖积累也是如此。这些观察结果部分可以通过高密度培养物中前体物质的膜转运减少来解释。使用低至500个稀疏接种的细胞，就可以在体外可靠地测定人成纤维细胞的合成活性。这种低细胞数测定对于研究复制寿命有限的单细胞克隆至关重要。
