Division of Genetics, Indian Agricultural Research Institute, New Delhi, India.
Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, India.
PLoS One. 2024 Oct 22;19(10):e0310003. doi: 10.1371/journal.pone.0310003. eCollection 2024.
Yellow mosaic disease (YMD) is a major constraint for the low productivity of mungbean, mainly in South Asia. Addressing this issue requires a comprehensive approach, integrating field and challenge inoculation evaluations to identify effective solutions. In this study, an infectious clone of Begomovirus vignaradiataindiaense (MYMIV) was developed to obtain a pure culture of the virus and to confirm resistance in mungbean plants exhibiting resistance under natural field conditions. The infectivity and efficiency of three Agrobacterium tumefaciens strains (EHA105, LBA4404, and GV3101) were evaluated using the susceptible mungbean genotype PS16. Additionally, a recombinant inbred line (RIL) population comprising 175 lines derived from Pusa Baisakhi (MYMIV susceptible) and PMR-1 (MYMIV resistant) cross was developed and assessed for YMD response. Among the tested Agrobacterium tumefaciens strains, EHA105 exhibited the highest infectivity (84.7%), followed by LBA4404 (54.7%) and GV3101 (9.80%). Field resistance was evaluated using the coefficient of infection (CI) and area under disease progress curve (AUDPC), identifying seven RILs with consistent resistant reactions (CI≤9) and low AUDPC (≤190). Upon challenge inoculation, six RILs exhibited resistance, while RIL92 displayed a resistance response, with infection occurring in less than 10% of plants after 24 to 29 days post inoculation (dpi). Despite some plants remaining asymptomatic, MYMIV presence was confirmed through specific PCR amplification of the MYMIV coat protein (AV1) gene. Quantitative PCR revealed a very low relative viral load (0.1-5.1% relative fold change) in asymptomatic RILs and the MYMIV resistant parent (PMR1) compared to the susceptible parent (Pusa Baisakhi). These findings highlight the potential utility of the developed infectious clone and the identified MYMIV-resistant RILs in future mungbean breeding programs aimed at cultivating MYMIV-resistant varieties.
黄脉花叶病（YMD）是降低绿豆产量的主要限制因素，主要发生在南亚地区。解决这个问题需要综合考虑，将田间和挑战接种评估结合起来，以确定有效的解决方案。在这项研究中，开发了一种印度舞毒蛾 Begomovirus vignaradiataindiaense（MYMIV）的传染性克隆，以获得病毒的纯培养物，并确认在自然田间条件下表现出抗性的绿豆植株的抗性。使用易感绿豆基因型 PS16 评估了三种根癌农杆菌菌株（EHA105、LBA4404 和 GV3101）的感染性和效率。此外，还开发了一个由 175 个系组成的重组自交系（RIL）群体，这些系来自 Pusa Baisakhi（MYMIV 敏感）和 PMR-1（MYMIV 抗性）杂交，并对 YMD 反应进行了评估。在测试的根癌农杆菌菌株中，EHA105 表现出最高的感染性（84.7%），其次是 LBA4404（54.7%）和 GV3101（9.80%）。使用感染系数（CI）和疾病进展曲线下面积（AUDPC）评估田间抗性，确定了 7 个具有一致抗性反应（CI≤9）和低 AUDPC（≤190）的 RIL。在挑战接种后，有 6 个 RIL 表现出抗性，而 RIL92 表现出抗性反应，在接种后 24 至 29 天内，感染不到 10%的植物。尽管一些植物仍然无症状，但通过特异性扩增 MYMIV 外壳蛋白（AV1）基因证实了 MYMIV 的存在。定量 PCR 显示，在无症状的 RIL 和抗性亲本（PMR1）中，相对病毒载量非常低（相对折叠变化的 0.1-5.1%），与易感亲本（Pusa Baisakhi）相比。这些发现突出了开发的传染性克隆和鉴定的 MYMIV 抗性 RIL 在未来绿豆育种计划中的潜在用途，旨在培育 MYMIV 抗性品种。
