Esteves Filipa, Brito David, Rajado Ana Teresa, Silva Nádia, Apolónio Joana, Roberto Vânia Palma, Andrade Raquel P, Calado Sofia, Faleiro Maria Leonor, Matos Carlos, Marques Nuno, Marreiros Ana, Nzwalo Hipólito, Pais Sandra, Palmeirim Isabel, Simãoa Sónia, Joaquim Natércia, Miranda Rui, Pêgas António, Raposo Daniela Marques, Sardo Ana, Araújo Inês, Nóbrega Clévio, Castelo-Branco Pedro, Bragança José
Algarve Biomedical Center Research Institute (ABC-RI), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; Algarve Biomedical Center (ABC), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal.
Algarve Biomedical Center Research Institute (ABC-RI), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; Algarve Biomedical Center (ABC), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; ABC Collaborative Laboratory, Association for Integrated Ageing and Rejuvenation Solutions (ABC CoLAB), 8100-735 Loulé, Portugal.
Stem Cell Res. 2024 Dec;81:103576. doi: 10.1016/j.scr.2024.103576. Epub 2024 Oct 15.
Human induced pluripotent stem cells (hiPSCs) hold promises to model and understand human diseases, including those associated with ageing. Here, we describe ABCRIi001-A, a hiPSC line generated from peripheral blood mononuclear cells (PBMCs) of a 79-year-old female enrolled in a study for development of an ageing score (ALFA Score). PBMCs were reprogrammed using three Sendai virus-based reprogramming vectors (hKOS, hc-Myc, and hKlf4). ABCRIi001-A showed normal morphology and karyotype, viral clearance, absence of genomic aberrations, and their pluripotency was confirmed by expression of pluripotency-related markers and their ability to differentiate into the three germ layers. ABCRIi001-A is valuable for ageing-related studies.
人诱导多能干细胞（hiPSC）有望用于模拟和理解人类疾病，包括与衰老相关的疾病。在此，我们描述了ABCRIi001-A，这是一种从一名79岁女性的外周血单核细胞（PBMC）中产生的hiPSC系，该女性参与了一项衰老评分（ALFA评分）开发研究。使用三种基于仙台病毒的重编程载体（hKOS、hc-Myc和hKlf4）对PBMC进行重编程。ABCRIi001-A表现出正常的形态和核型，病毒清除，无基因组畸变，并且通过多能性相关标志物的表达及其分化为三个胚层的能力证实了其多能性。ABCRIi001-A对于衰老相关研究具有重要价值。
