Lefevre Alexis, Brandi Cristian, De Ninno Adele, Ruggiero Filippo, Verona Enrico, Gauthier Michaël, Bisegna Paolo, Bolopion Aude, Caselli Federica
Université de Franche-Comté, CNRS, SUPMICROTECH-ENSMM, Institute FEMTO-ST, F-25000 Besançon, France.
Department of Civil Engineering and Computer Science, University of Rome Tor Vergata, Rome, Italy.
Lab Chip. 2024 Nov 5;24(22):5145-5154. doi: 10.1039/d4lc00622d.
This work presents an innovative all-electrical platform for selective single-particle manipulation. The platform combines microfluidic impedance cytometry for label-free particle characterization and dielectrophoresis for contactless multi-way particle separation. The microfluidic chip has a straightforward coplanar electrode layout and no particle pre-focusing mechanism is required. An original online algorithm analyzes the impedance signals of each incoming particle and regulates in real time the dielectrophoretic voltages according to a desired control logic. As a proof-of-concept, three operation modes are demonstrated on a mixture of 8, 10, and 12 μm diameter beads: (i) particle position swapping across the channel axis, irrespective of particle size, (ii) size-based particle separation, irrespective of particle position, and (iii) sorting of a selected sequence of particles. As a perspective, the versatility of impedance cytometry and dielectrophoresis, and the possibility of configuring alternative control logics, hold promise for advanced particle and cell manipulation.
这项工作展示了一个用于选择性单粒子操控的创新型全电平台。该平台将用于无标记粒子表征的微流控阻抗细胞术和用于非接触式多路粒子分离的介电泳相结合。微流控芯片具有简单的共面电极布局，无需粒子预聚焦机制。一种原创的在线算法分析每个进入粒子的阻抗信号，并根据所需的控制逻辑实时调节介电泳电压。作为概念验证，在直径为8、10和12μm的珠子混合物上展示了三种操作模式：(i) 粒子在通道轴上的位置交换，与粒子大小无关；(ii) 基于大小的粒子分离，与粒子位置无关；(iii) 对选定粒子序列进行分选。展望未来，阻抗细胞术和介电泳的多功能性以及配置替代控制逻辑的可能性，为先进的粒子和细胞操控带来了希望。
