Makemson J C
J Bacteriol. 1986 Feb;165(2):461-6. doi: 10.1128/jb.165.2.461-466.1986.
Oxygen uptake due to luciferase in two luminous Vibrio species was estimated in vivo by utilizing inhibitors having specificities for luciferase (decanol) and cytochromes (cyanide). Cyanide titration of respiration revealed a component of oxygen uptake less sensitive to cyanide which was completely inhibitable by low concentrations of decanol. From this it was estimated that in vivo luciferase is responsible for less than 12% (Vibrio harveyi) or 20% (Vibrio fischeri) of the total respiration. From these data in vivo bioluminescent quantum yields are estimated to be not lower than 1.7 and 2.6%, respectively.
通过使用对荧光素酶(癸醇)和细胞色素(氰化物)具有特异性的抑制剂,在体内估计了两种发光弧菌中荧光素酶引起的氧气摄取。呼吸作用的氰化物滴定显示,存在一部分对氰化物不太敏感的氧气摄取成分,低浓度的癸醇可完全抑制该成分。据此估计,在体内荧光素酶对总呼吸作用的贡献小于12%(哈维氏弧菌)或20%(费氏弧菌)。根据这些数据,体内生物发光量子产率估计分别不低于1.7%和2.6%。