Vysotskiĭ E S, Zavoruev V V, Mezhevikin V V
Mikrobiologiia. 1981 Nov-Dec;50(6):985-91.
The effect of phenobarbital on the luminescent system of Beneckea harveyi was studied. The inhibition of luminescence with phenobarbital was shown to be due to a disorder in the synthesis of an aldehyde factor, the endogenous substrate of bacterial luciferase. Upon the action of phenobarbital, the bacterium acquires the properties of "aldehyde" mutants, i. e. their luminescence is stimulated with exogenous decyl aldehyde. The luminescence of the cells was also stimulated with long-chain aldehydes, fatty acids and their analogues: apparently, the aldehyde factor is formed via incorporation of an oxygen atom into the terminal methyl of a saturated fatty acid or its analogue. Phenobarbital has no effect on the bacterial growth; however, it increases the content of luciferase in the culture. The results suggest that phenobarbital is not a direct inductor of luciferase synthesis. Possibly, the stimulating action of phenobarbital involves the inhibition of synthesis of the aldehyde factor and, consequently, an increase in the concentration of intermediate products of its synthesis.
研究了苯巴比妥对哈维氏贝内克氏菌发光系统的影响。结果表明，苯巴比妥对发光的抑制是由于醛因子（细菌荧光素酶的内源性底物）合成紊乱所致。在苯巴比妥的作用下，细菌获得了“醛”突变体的特性，即其发光可被外源性癸醛刺激。细胞的发光也可被长链醛、脂肪酸及其类似物刺激：显然，醛因子是通过将一个氧原子掺入饱和脂肪酸或其类似物的末端甲基而形成的。苯巴比妥对细菌生长没有影响；然而，它会增加培养物中荧光素酶的含量。结果表明，苯巴比妥不是荧光素酶合成的直接诱导剂。苯巴比妥的刺激作用可能涉及对醛因子合成的抑制，从而导致其合成中间产物浓度的增加。
