Patel D J, Shapiro L, Hare D
J Biol Chem. 1986 Jan 25;261(3):1223-9.
The nonexchangeable base and sugar protons of the octanucleotide d(G-G-A-A-T-T-C-C) have been assigned by two-dimensional correlated (COSY) and nuclear Overhauser effect (NOESY) methods in aqueous solution. The assignments are based on distance connectivities of less than 4.5 A established from NOE effects between base and sugar protons on the same strand and occasionally between strands, as well as, coupling connectivities within the protons on each sugar ring. We observe the NOEs to exhibit directionality and are consistent with the d(G-G-A-A-T-T-C-C) duplex adopting a right-handed helix in solution. The relative magnitude of the NOEs between base and sugar H2' protons of the same and 5'-adjacent sugars characterizes the AATT segment to the B-helix type in solution.
八聚体d（G-G-A-A-T-T-C-C）的非交换性碱基和糖质子已通过二维相关（COSY）和核Overhauser效应（NOESY）方法在水溶液中进行了归属。这些归属基于通过同一条链上以及偶尔链间碱基和糖质子之间的NOE效应建立的小于4.5埃的距离连接性，以及每个糖环上质子之间的耦合连接性。我们观察到NOE表现出方向性，并且与溶液中采用右手螺旋的d（G-G-A-A-T-T-C-C）双链体一致。相同和5'-相邻糖的碱基与糖H2'质子之间NOE的相对大小将溶液中的AATT片段表征为B-螺旋类型。
