Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022 València, Spain.
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Instituto de Salud Carlos III, 28029 Madrid, Spain.
Nano Lett. 2024 Nov 6;24(44):14050-14057. doi: 10.1021/acs.nanolett.4c04022. Epub 2024 Oct 23.
Inspired by nature, the development of artificial micro/nanosystems capable of communicating has become an emergent topic in nanotechnology, synthetic biology, and related areas. However, the demonstration of actual applications still has to come. Here, we demonstrate how chemical communication between micro- and nanoparticles can be used for the design of sensing systems. To realize this, we synergistically combine two different types of particles: i.e., giant unilamellar vesicles (GUVs) as senders and gated mesoporous nanoparticles as receivers. The use of engineered GUVs allows the detection of analytes based on responsive membranes, while the use of gated nanoparticles allows a straightforward application on test strips with smartphone-based detection. In addition, we demonstrate that the combined communication system exhibits signal amplification and its application in real samples employing the bacterial toxin α-hemolysin as target analyte. Altogether, our report presents a new route for engineering sensing systems based on the combination of communicative micro/nanoparticles.
受自然启发，能够进行通信的人工微/纳系统的开发已经成为纳米技术、合成生物学和相关领域的一个新兴课题。然而，实际应用的演示还有待实现。在这里，我们展示了微纳米粒子之间的化学通信如何用于设计传感系统。为此，我们协同结合了两种不同类型的粒子：即作为发送器的巨大单层囊泡（GUV）和作为接收器的门控介孔纳米粒子。工程化 GUV 的使用允许基于响应膜检测分析物，而门控纳米粒子的使用允许在带有智能手机检测的试纸上直接应用。此外，我们证明了组合通信系统具有信号放大功能，并将其应用于以细菌毒素 α-溶血素为目标分析物的实际样品中。总的来说，我们的报告提出了一种基于通信微/纳粒子组合的工程传感系统的新途径。
