Mukwaya Vincent, Mann Stephen, Dou Hongjing
State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.
Max Planck Bristol Centre for Minimal Biology and Centre for Protolife Research, School of Chemistry, University of Bristol, Bristol, BS8 1TS, UK.
Commun Chem. 2021 Nov 25;4(1):161. doi: 10.1038/s42004-021-00597-w.
Although the complexity of synthetic cells has continued to increase in recent years, chemical communication between protocell models and living organisms remains a key challenge in bottom-up synthetic biology and bioengineering. In this Review, we discuss how communication channels and modes of signal processing can be established between living cells and cytomimetic agents such as giant unilamellar lipid vesicles, proteinosomes, polysaccharidosomes, polymer-based giant vesicles and membrane-less coacervate micro-droplets. We describe three potential modes of chemical communication in consortia of synthetic and living cells based on mechanisms of distributed communication and signal processing, physical embodiment and nested communication, and network-based contact-dependent communication. We survey the potential for applying synthetic cell/living cell communication systems in biomedicine, including the in situ production of therapeutics and development of new bioreactors. Finally, we present a short summary of our findings.
尽管近年来合成细胞的复杂性持续增加，但原细胞模型与活生物体之间的化学通讯仍是自下而上合成生物学和生物工程领域的一项关键挑战。在本综述中，我们讨论了如何在活细胞与细胞模拟物（如巨型单层脂质囊泡、蛋白体、多糖体、基于聚合物的巨型囊泡和无膜凝聚微滴）之间建立通讯渠道和信号处理模式。我们基于分布式通讯和信号处理机制、物理体现和嵌套通讯以及基于网络的接触依赖性通讯，描述了合成细胞与活细胞联合体中三种潜在的化学通讯模式。我们探讨了在生物医学中应用合成细胞/活细胞通讯系统的潜力，包括原位生产治疗药物和开发新型生物反应器。最后，我们简要总结了我们的研究结果。
