Institute for Advanced Study, Chengdu University, Chengdu 610106, China; School of Food and Biological Engineering, Chengdu University, Chengdu 610106, China.
Department of Laboratory Medicine, West China Hospital, Sichuan University, Sichuan Clinical Research Center for Laboratory Medicine, Clinical Laboratory Medicine Research Center of West China Hospital, Chengdu 610041, China.
Food Chem. 2025 Feb 1;464(Pt 2):141715. doi: 10.1016/j.foodchem.2024.141715. Epub 2024 Oct 19.
Revealing the interaction modes between nanoscale metal-organic frameworks (NMOFs) and food matrix is crucial for functional release but it still remains largely unknown to date. This study specifically focused on the milk protein adsorption mechanism of NMOFs using UiO66/UiO66-NH and β-lactoglobulin (β-LG) as models. UiO66 and UiO66-NH quenched the fluorescence of β-LG via static mechanism. Due to the enhanced electrostatic forces caused by NH, UiO66-NH-β-LG (2.83 × 10 mol·L) exhibited higher binding constant than UiO66-β-LG (2.61 × 10 mol·L), while UiO66 with higher hydrophobicity adsorbed more β-LG. The defects of UiO influenced the binding sites on the β-LG, and the higher the defect degree, the higher the binding energy. For the stability of the system, the H-bonding between UiO66 and SER30/PRO38, and the hydrophobic interaction between UiO66-NH and LYS101 played important roles. Furthermore, the secondary structure content of β-LG changed after interacting with both UiO, resulting in reduced density of β-LG.
揭示纳米尺度金属有机骨架（NMOFs）与食物基质之间的相互作用模式对于功能性释放至关重要，但目前仍知之甚少。本研究以 UiO66/UiO66-NH 和 β-乳球蛋白（β-LG）为模型，专门研究了 NMOFs 对牛奶蛋白的吸附机制。UiO66 和 UiO66-NH 通过静态机制猝灭β-LG 的荧光。由于 NH 引起的静电力增强，UiO66-NH-β-LG（2.83×10^-4mol·L）表现出比 UiO66-β-LG（2.61×10^-4mol·L）更高的结合常数，而具有更高疏水性的 UiO66 吸附更多的β-LG。UiO 的缺陷影响β-LG 的结合位点，缺陷程度越高，结合能越高。对于体系的稳定性，UiO66 与 SER30/PRO38 之间的氢键以及 UiO66-NH 与 LYS101 之间的疏水相互作用发挥了重要作用。此外，β-LG 的二级结构含量在与两种 UiO 相互作用后发生变化，导致β-LG 的密度降低。
