Okada Tomoyuki, Koike Yuriko, Shoji Tadashi, Sutou Kazuhisa
Gunma Prefectural Food Safety Research Center.
Gunma Prefectural Institute of Public Health and Environmental Sciences.
Shokuhin Eiseigaku Zasshi. 2024;65(4):95-100. doi: 10.3358/shokueishi.65.95.
We developed a simultaneous determination method for the environmentally persistent organochlorine pesticides aldrin/dieldrin, γ-BHC, DDT, endrin, and heptachlor in beef products. In the method, we adopted incremental reduction for sample collection in order to improve the uniformity of the samples. In incremental reduction, a sample was ground and spread to an even thickness, divided into sections, and collected in an equal amount from each section. After extraction with an acetonitrile/ethanol mixture (1 : 1 v/v), sample clean-up was performed by refrigerated centrifu-gation and solid-phase extraction. This process was easy and did not require gel permeation chromatography. The sample was analyzed by GC-MS. A validation study of the development method showed that the tested pesticides met the target values indicated in the validity evaluation guideline.
我们开发了一种同时测定牛肉制品中环境持久性有机氯农药艾氏剂/狄氏剂、γ-六六六、滴滴涕、异狄氏剂和七氯的方法。在该方法中，我们采用增量缩减法进行样品采集，以提高样品的均匀性。在增量缩减过程中，将样品研磨并铺展成均匀的厚度，分成若干部分，然后从每个部分等量采集。用乙腈/乙醇混合物（1:1 v/v）萃取后，通过冷藏离心和固相萃取进行样品净化。该过程简便，无需凝胶渗透色谱法。样品通过气相色谱-质谱联用仪进行分析。对所开发方法的验证研究表明，所检测的农药符合有效性评估指南中规定的目标值。
