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弓形虫WH3 Δrop18作为一种针对急性和慢性弓形虫病的减毒活疫苗。

Toxoplasma WH3 Δrop18 acts as a live attenuated vaccine against acute and chronic toxoplasmosis.

作者信息

Wang Cong, Fu Shengnan, Yu Xin, Zhou Hang, Zhang Famin, Song Lingling, Zhao Ji, Yang Yun, Du Jianbing, Luo Qingli, Shen Jilong, Yu Li

机构信息

Department of Microbiology and Parasitology, Anhui Province key Laboratory of Zoonoses and The Provincial Key Laboratory of Zoonoses of High Institutions Anhui School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province, PR China.

Department of Clinical Laboratory, The Second People's Hospital of Hefei, Hefei, Anhui Province, PR China.

出版信息

NPJ Vaccines. 2024 Oct 23;9(1):197. doi: 10.1038/s41541-024-00996-9.

DOI:10.1038/s41541-024-00996-9
PMID:39443531
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11500380/
Abstract

Toxoplasma gondii is a significant zoonotic pathogen of toxoplasmosis in humans and animals. Here a live attenuated Toxoplasma vaccine of WH3 Δrop18 was developed. The results showed that all mice vaccinated with WH3 Δrop18 were able to survive when challenge with various strains of Toxoplasma, including RH (type I), ME49 (type II), WH3 or WH6 (type Chinese 1). No cysts, if few, in the brain of the vaccinated animals were seen after challenge with cyst forming strains of ME49 or WH6. Vaccination with the WH3 Δrop18 triggered a strong immune response, including significantly increased level of the cytokines (IFN-γ, IL-12, TNF-α and IL-10) and the activation of CD4 and CD8 T-lymphocytes and long term of specific antibodies against Toxoplasma. Our results strongly indicate that vaccine of WH3 Δrop18 might provide effective immune protection against a wide range strains of Toxoplasma infections and be a promising live attenuated vaccine candidate.

摘要

刚地弓形虫是人和动物弓形虫病的一种重要人兽共患病原体。在此研发了一种WH3 Δrop18减毒活弓形虫疫苗。结果显示，用WH3 Δrop18疫苗接种的所有小鼠在用包括RH（I型）、ME49（II型）、WH3或WH6（中国1型）等各种弓形虫菌株攻击时均能存活。在用ME49或WH6的包囊形成菌株攻击后，接种动物的大脑中未见包囊，即便有也很少。用WH3 Δrop18疫苗接种引发了强烈的免疫反应，包括细胞因子（IFN-γ、IL-12、TNF-α和IL-10）水平显著升高，CD4和CD8 T淋巴细胞活化，以及针对弓形虫的特异性抗体长期存在。我们的结果有力地表明，WH3 Δrop18疫苗可能对多种弓形虫菌株感染提供有效的免疫保护，是一种有前景的减毒活疫苗候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c83f/11500380/5875f7b14a67/41541_2024_996_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c83f/11500380/68bc3b65de24/41541_2024_996_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c83f/11500380/efb6aed8608b/41541_2024_996_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c83f/11500380/ae8343e6513b/41541_2024_996_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c83f/11500380/7e303117bab6/41541_2024_996_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c83f/11500380/fec868757dea/41541_2024_996_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c83f/11500380/91b9c52b379e/41541_2024_996_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c83f/11500380/0f793995d8f0/41541_2024_996_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c83f/11500380/a29ba871e76f/41541_2024_996_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c83f/11500380/5875f7b14a67/41541_2024_996_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c83f/11500380/68bc3b65de24/41541_2024_996_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c83f/11500380/efb6aed8608b/41541_2024_996_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c83f/11500380/ae8343e6513b/41541_2024_996_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c83f/11500380/7e303117bab6/41541_2024_996_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c83f/11500380/fec868757dea/41541_2024_996_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c83f/11500380/91b9c52b379e/41541_2024_996_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c83f/11500380/0f793995d8f0/41541_2024_996_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c83f/11500380/a29ba871e76f/41541_2024_996_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c83f/11500380/5875f7b14a67/41541_2024_996_Fig9_HTML.jpg

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本文引用的文献

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Infect Dis Poverty. 2023 Jun 15;12(1):60. doi: 10.1186/s40249-023-01109-9.
2
Live-attenuated ME49Δcdpk3 strain of Toxoplasma gondii protects against acute and chronic toxoplasmosis.刚地弓形虫减毒活疫苗ME49Δcdpk3株可预防急性和慢性弓形虫病。
NPJ Vaccines. 2022 Aug 20;7(1):98. doi: 10.1038/s41541-022-00518-5.
3
Protective Effect Against Toxoplasmosis in Mice Vaccinated With Recombinant MIF, CDPK3, and 14-3-3 Protein Cocktail Vaccine.
用重组MIF、CDPK3和14-3-3蛋白混合疫苗接种的小鼠对弓形虫病的保护作用
Front Immunol. 2021 Dec 22;12:755792. doi: 10.3389/fimmu.2021.755792. eCollection 2021.
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A Novel Combined DNA Vaccine Encoding Toxoplasma gondii SAG1 and ROP18 Provokes Protective Immunity Against a Lethal Challenge in Mice.一种编码刚地弓形虫表面抗原1（SAG1）和棒状体蛋白18（ROP18）的新型联合DNA疫苗可激发小鼠对致死性攻击的保护性免疫。
Acta Parasitol. 2021 Dec;66(4):1387-1395. doi: 10.1007/s11686-021-00415-2. Epub 2021 May 21.
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:Δ Live Attenuated Strain Induces Protective Immunity Against Acute and Chronic Toxoplasmosis.减毒活菌株诱导针对急性和慢性弓形虫病的保护性免疫。
Front Microbiol. 2021 Mar 11;12:619335. doi: 10.3389/fmicb.2021.619335. eCollection 2021.
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Global status of Toxoplasma gondii infection: systematic review and prevalence snapshots.全球弓形虫感染状况：系统评价和流行率快照。
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