• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

丙酸杆菌 MJ2 衍生的细胞外囊泡抑制 RANKL 诱导的破骨细胞生成并改善胶原诱导的类风湿关节炎。

Propionibacterium freudenreichii MJ2-derived extracellular vesicles inhibit RANKL-induced osteoclastogenesis and improve collagen-induced rheumatoid arthritis.

机构信息

Department of Integrated Biomedical and Life Sciences, Graduate School, Korea University, Seoul, 02841, Republic of Korea.

School of Biosystems and Biomedical Sciences, Korea University, Seoul, 02841, Republic of Korea.

出版信息

Sci Rep. 2024 Oct 23;14(1):24973. doi: 10.1038/s41598-024-76911-y.

DOI:10.1038/s41598-024-76911-y
PMID:39443658
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11500175/
Abstract

Rheumatoid arthritis causes excessive bone loss by stimulating osteoclast differentiation. Extracellular vesicles are valuable disease markers, conveyors of distant cell-to-cell communication, and carriers for drug delivery. The aim of this study was to investigate the anti-osteoclastogenic effects of extracellular vesicles derived from dairy Propionibacterium freudenreichii MJ2 (PFEVs) and the improvement effect of PFEVs on collagen-induced arthritis (CIA) animal model. PFEVs were observed by scanning electron microscopy, transmission electron microscopy, nanoparticle tracking analysis, and LC-MS/MS. The inhibitory activity of PFEVs against receptor activator of nuclear factor kappa-B ligand (RANKL)-induced osteoclast differentiation was investigated in RAW 264.7 cells. PFEVs significantly decreased the expression levels of genes and proteins related to osteoclast differentiation. PFEVs decreased RANK-RANKL binding. In a CIA mouse model, PFEVs treatment significantly reduced arthritis scores and collagen-specific immunoglobulins. PFEVs treatment also reduced pro-inflammatory cytokines and increased anti-inflammatory cytokines. The anti-inflammatory effects were confirmed by H&E staining, and PFEVs treatment inhibited osteoclastogenesis in the CIA mouse model. In conclusion, PFEVs inhibited osteoclast differentiation by inhibiting RANK-RANKL signaling, thereby decreasing the expression of osteoclast differentiation-related genes. PFEVs also improved collagen-induced arthritis by inhibiting inflammation and osteoclastogenesis.

摘要

类风湿关节炎通过刺激破骨细胞分化导致过度的骨质流失。细胞外囊泡是有价值的疾病标志物，是远距离细胞间通讯的载体，也是药物递送的载体。本研究旨在探讨源自乳丙酸杆菌 MJ2（PFEVs）的细胞外囊泡对破骨细胞生成的抑制作用及其对胶原诱导性关节炎（CIA）动物模型的改善作用。通过扫描电子显微镜、透射电子显微镜、纳米颗粒跟踪分析和 LC-MS/MS 观察 PFEVs。在 RAW 264.7 细胞中研究了 PFEVs 对核因子 kappa-B 配体（RANKL）诱导的破骨细胞分化的抑制活性。PFEVs 显著降低了与破骨细胞分化相关的基因和蛋白的表达水平。PFEVs 减少了 RANK-RANKL 结合。在 CIA 小鼠模型中，PFEVs 治疗显著降低了关节炎评分和胶原特异性免疫球蛋白。PFEVs 治疗还降低了促炎细胞因子并增加了抗炎细胞因子。H&E 染色证实了抗炎作用，PFEVs 治疗抑制了 CIA 小鼠模型中的破骨细胞生成。总之，PFEVs 通过抑制 RANK-RANKL 信号通路抑制破骨细胞分化，从而降低破骨细胞分化相关基因的表达。PFEVs 还通过抑制炎症和破骨细胞生成改善胶原诱导性关节炎。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4999/11500175/90dc616e314c/41598_2024_76911_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4999/11500175/f92c20bc9d20/41598_2024_76911_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4999/11500175/a8cb00ca1056/41598_2024_76911_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4999/11500175/766716f67d6b/41598_2024_76911_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4999/11500175/d8ba80a1443c/41598_2024_76911_Fig4a_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4999/11500175/32d48cd0aabf/41598_2024_76911_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4999/11500175/c17e94cc94d3/41598_2024_76911_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4999/11500175/90dc616e314c/41598_2024_76911_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4999/11500175/f92c20bc9d20/41598_2024_76911_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4999/11500175/a8cb00ca1056/41598_2024_76911_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4999/11500175/766716f67d6b/41598_2024_76911_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4999/11500175/d8ba80a1443c/41598_2024_76911_Fig4a_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4999/11500175/32d48cd0aabf/41598_2024_76911_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4999/11500175/c17e94cc94d3/41598_2024_76911_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4999/11500175/90dc616e314c/41598_2024_76911_Fig7_HTML.jpg

相似文献

1
Propionibacterium freudenreichii MJ2-derived extracellular vesicles inhibit RANKL-induced osteoclastogenesis and improve collagen-induced rheumatoid arthritis.丙酸杆菌 MJ2 衍生的细胞外囊泡抑制 RANKL 诱导的破骨细胞生成并改善胶原诱导的类风湿关节炎。
Sci Rep. 2024 Oct 23;14(1):24973. doi: 10.1038/s41598-024-76911-y.
2
Microbial chaperonin 60 inhibits osteoclast differentiation by interfering with RANK/RANKL binding and overexpression of lipocalin2.微生物热休克蛋白 60 通过干扰 RANK/RANKL 结合和脂联素 2 的过表达抑制破骨细胞分化。
Biochim Biophys Acta Mol Cell Res. 2024 Dec;1871(8):119850. doi: 10.1016/j.bbamcr.2024.119850. Epub 2024 Sep 19.
3
Inhibits RANKL-Induced Osteoclast Differentiation and Ameliorates Rheumatoid Arthritis in Collagen-Induced Arthritis Mice.抑制RANKL诱导的破骨细胞分化并改善胶原诱导性关节炎小鼠的类风湿性关节炎。
Microorganisms. 2021 Dec 27;10(1):48. doi: 10.3390/microorganisms10010048.
4
Surface proteins of Propionibacterium freudenreichii MJ2 inhibit RANKL-induced osteoclast differentiation by lipocalin-2 upregulation and lipocalin-2-mediated NFATc1 inhibition.丙酸杆菌表面蛋白通过上调脂联素 2 和脂联素 2 介导的 NFATc1 抑制抑制 RANKL 诱导的破骨细胞分化。
Sci Rep. 2023 Sep 20;13(1):15644. doi: 10.1038/s41598-023-42944-y.
5
Asperosaponin VI protects against bone destructions in collagen induced arthritis by inhibiting osteoclastogenesis.柴胡皂苷 VI 通过抑制破骨细胞生成来防治胶原诱导性关节炎中的骨破坏。
Phytomedicine. 2019 Oct;63:153006. doi: 10.1016/j.phymed.2019.153006. Epub 2019 Jul 2.
6
Fengshi Qutong capsule ameliorates bone destruction of experimental rheumatoid arthritis by inhibiting osteoclastogenesis.风蚀祛痛胶囊通过抑制破骨细胞生成改善实验性类风湿性关节炎的骨破坏。
J Ethnopharmacol. 2022 Jan 10;282:114602. doi: 10.1016/j.jep.2021.114602. Epub 2021 Sep 4.
7
TWEAK promotes osteoclastogenesis in rheumatoid arthritis.TWEAK 促进类风湿关节炎中的破骨细胞生成。
Am J Pathol. 2013 Sep;183(3):857-67. doi: 10.1016/j.ajpath.2013.05.027. Epub 2013 Jul 8.
8
Boldine isolated from Litsea cubeba inhibits bone resorption by suppressing the osteoclast differentiation in collagen-induced arthritis.从山鸡椒中分离出的荜澄茄素通过抑制胶原诱导性关节炎中的破骨细胞分化来抑制骨吸收。
Int Immunopharmacol. 2017 Oct;51:114-123. doi: 10.1016/j.intimp.2017.08.013. Epub 2017 Aug 18.
9
The inhibitory effects of a RANKL-binding peptide on articular and periarticular bone loss in a murine model of collagen-induced arthritis: a bone histomorphometric study.一种RANKL结合肽对胶原诱导性关节炎小鼠模型关节及关节周围骨质流失的抑制作用：一项骨组织形态计量学研究
Arthritis Res Ther. 2015 Sep 12;17(1):251. doi: 10.1186/s13075-015-0753-8.
10
Iguratimod inhibits osteoclastogenesis by modulating the RANKL and TNF-α signaling pathways.艾拉莫德通过调节RANKL和TNF-α信号通路抑制破骨细胞生成。
Int Immunopharmacol. 2021 Jan;90:107219. doi: 10.1016/j.intimp.2020.107219. Epub 2020 Dec 9.

引用本文的文献

1
Probiotic-derived extracellular vesicles: the next breakthrough in postbiotics for rheumatoid arthritis.益生菌衍生的细胞外囊泡：类风湿性关节炎后生元的下一个突破。
Front Immunol. 2025 Aug 7;16:1620185. doi: 10.3389/fimmu.2025.1620185. eCollection 2025.
2
Dairy Propionibacteria: Probiotic Properties and Their Molecular Bases.乳制品丙酸杆菌：益生菌特性及其分子基础
Biomolecules. 2025 Jun 17;15(6):886. doi: 10.3390/biom15060886.

本文引用的文献

1
The functions and applications of extracellular vesicles derived from Mycobacterium tuberculosis.结核分枝杆菌来源的细胞外囊泡的功能和应用。
Biomed Pharmacother. 2023 Dec;168:115767. doi: 10.1016/j.biopha.2023.115767. Epub 2023 Oct 20.
2
Extracellular nanovesicles produced by Bacillus licheniformis: A potential anticancer agent for breast and lung cancer.地衣芽孢杆菌产生的细胞外纳米囊泡：一种用于乳腺癌和肺癌的潜在抗癌剂。
Microb Pathog. 2023 Dec;185:106396. doi: 10.1016/j.micpath.2023.106396. Epub 2023 Oct 19.
3
From trash to treasure: the role of bacterial extracellular vesicles in gut health and disease.
从垃圾到珍宝：细菌细胞外囊泡在肠道健康和疾病中的作用。
Front Immunol. 2023 Sep 29;14:1274295. doi: 10.3389/fimmu.2023.1274295. eCollection 2023.
4
Roles of bacterial extracellular vesicles in systemic diseases.细菌细胞外囊泡在全身性疾病中的作用。
Front Microbiol. 2023 Sep 28;14:1258860. doi: 10.3389/fmicb.2023.1258860. eCollection 2023.
5
Surface proteins of Propionibacterium freudenreichii MJ2 inhibit RANKL-induced osteoclast differentiation by lipocalin-2 upregulation and lipocalin-2-mediated NFATc1 inhibition.丙酸杆菌表面蛋白通过上调脂联素 2 和脂联素 2 介导的 NFATc1 抑制抑制 RANKL 诱导的破骨细胞分化。
Sci Rep. 2023 Sep 20;13(1):15644. doi: 10.1038/s41598-023-42944-y.
6
Neutrophil extracellular traps mediate bone erosion in rheumatoid arthritis by enhancing RANKL-induced osteoclastogenesis.中性粒细胞胞外诱捕网通过增强 RANKL 诱导的破骨细胞生成来介导类风湿关节炎中的骨侵蚀。
Br J Pharmacol. 2024 Feb;181(3):429-446. doi: 10.1111/bph.16227. Epub 2023 Sep 28.
7
Osteoclast differentiation in rheumatoid arthritis.类风湿关节炎中的破骨细胞分化
Immunol Med. 2024 Mar;47(1):6-11. doi: 10.1080/25785826.2023.2220931. Epub 2023 Jun 13.
8
Anti-Inflammatory Effect of Clostridium butyricum-Derived Extracellular Vesicles in Ulcerative Colitis: Impact on Host microRNAs Expressions and Gut Microbiome Profiles.丁酸梭菌来源的细胞外囊泡在溃疡性结肠炎中的抗炎作用：对宿主 microRNAs 表达和肠道微生物组谱的影响。
Mol Nutr Food Res. 2023 Jul;67(13):e2200884. doi: 10.1002/mnfr.202200884. Epub 2023 Jun 11.
9
Rheumatoid Arthritis - Common Origins, Divergent Mechanisms.类风湿关节炎——共同起源，不同机制。
N Engl J Med. 2023 Feb 9;388(6):529-542. doi: 10.1056/NEJMra2103726.
10
Extracellular vesicles: The next generation in gene therapy delivery.细胞外囊泡：基因治疗传递的下一代。
Mol Ther. 2023 May 3;31(5):1225-1230. doi: 10.1016/j.ymthe.2023.01.021. Epub 2023 Jan 25.