Oliveira Jonatas Silva de, Ribas Beatriz Ribeiro, Ferro Amanda Costa, Tasso Camilla Olga, Camargo Rafaelly, Cavalheiro Alberto José, Jorge Janaina Habib
School of Dentistry, Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), São Paulo, Brazil.
Institute of Chemistry, Department of Organic Chemistry, São Paulo State University (UNESP), São Paulo, Brazil.
Biofouling. 2024 Nov;40(10):831-846. doi: 10.1080/08927014.2024.2418466. Epub 2024 Oct 24.
Extracts of species have been shown to reduce biofilms, demonstrating their antimicrobial effects. The extracts can be fractionated to optimize their potential. In this study, we evaluated the antimicrobial activity of fractions against planktonic cells and biofilms of and . Four fractions were prepared: 100% hexane, acetate/hexane 1:1, 100% ethyl acetate, and water. The effect of the fractions on planktonic cells was assessed by counting the colony-forming units per milliliter (CFU/mL). Biofilm tests included CFU/mL, cell metabolic activity, and qualitative analysis using confocal laser scanning microscopy (CLSM). Results were analyzed by the Mann-Whitney U test (α = 0.05). The fractions contained lipophilic constituents, styrylpyrones, glycosylated flavonoids, and alkaloids. Acetate/hexane (1:1) and 100% ethyl acetate fractions reduced the CFU/mL of planktonic fractions did not affect planktonic . For biofilms, the fractions reduced the CFU/mL (from 2-5 logs) and cell metabolic activity (approximately 80% reduction in a single-species biofilm). CLSM showed the fractions reduced microorganism viability and damaged the extracellular matrix of biofilms. We conclude that the acetate/hexane 1:1 and 100% ethyl acetate fractions exhibit antimicrobial effects against biofilms.
已证明某些物种的提取物可减少生物膜，显示出其抗菌作用。这些提取物可进行分级分离以优化其潜力。在本研究中，我们评估了各分级提取物对金黄色葡萄球菌和大肠杆菌的浮游细胞及生物膜的抗菌活性。制备了四个分级提取物：100%己烷、乙酸乙酯/己烷1:1、100%乙酸乙酯和水。通过计算每毫升菌落形成单位（CFU/mL）来评估各分级提取物对浮游细胞的影响。生物膜测试包括CFU/mL、细胞代谢活性以及使用共聚焦激光扫描显微镜（CLSM）进行定性分析。结果采用曼-惠特尼U检验进行分析（α = 0.05）。这些分级提取物含有亲脂性成分、苯乙烯基吡喃酮、糖基化黄酮类化合物和生物碱。乙酸乙酯/己烷（1:1）和100%乙酸乙酯分级提取物降低了金黄色葡萄球菌浮游细胞的CFU/mL；各分级提取物对大肠杆菌浮游细胞无影响。对于生物膜，这些分级提取物降低了CFU/mL（降低2 - 5个对数）和细胞代谢活性（在单物种生物膜中降低约80%）。CLSM显示这些分级提取物降低了微生物活力并破坏了生物膜的细胞外基质。我们得出结论，乙酸乙酯/己烷1:1和100%乙酸乙酯分级提取物对生物膜具有抗菌作用。
