Beaulieu L, Valet J P, Beaudoin J, Boulet A, Jobin M, Perelmutter L, Hébert J
J Immunol Methods. 1986 Jan 22;86(1):131-8. doi: 10.1016/0022-1759(86)90276-0.
Radioallergosorbent test (RAST) for the measurement of IgE antibodies has been introduced more than 15 years ago and a number of technical modifications have since improved its sensitivity and reproducibility. The test has been applied to the diagnosis of allergy and to determine changes in the levels of IgE antibodies following immunotherapy. However, specific IgG antibodies are raised during such a therapy and can interfere with the RAST. We have developed a reverse enzymoallergosorbent test (REAST) where microtiter plates are first coated with a purified polyclonal anti-IgE antibody, then with the serum to test and finally with peroxidase-labeled antigen. This assay is antigen specific as shown by the significant inhibition of binding of the labeled antigen in presence of unlabeled specific antigen (greater than 95%) and the absence of inhibition in presence of irrelevant antigens. The values found in atopic patients (85 subjects) were significantly higher than in the non-atopic donors (35 subjects) (1.14 U +/- 1.20 vs. 0.01 U +/- 0.02, P less than 0.0005) and there was a good correlation with the Pharmacia RAST (P less than 0.0005). The levels of specific IgE by both REAST and RAST correlated well with the clinical symptomatology.
用于测量IgE抗体的放射变应原吸附试验（RAST）在15年多以前就已问世，自那时起，一些技术改进提高了其灵敏度和可重复性。该试验已应用于过敏症的诊断以及确定免疫治疗后IgE抗体水平的变化。然而，在这种治疗过程中会产生特异性IgG抗体，它们会干扰RAST。我们开发了一种反向酶变应原吸附试验（REAST），在微量滴定板上首先包被纯化的多克隆抗IgE抗体，然后加入待检测血清，最后加入过氧化物酶标记的抗原。如在未标记的特异性抗原存在时标记抗原的结合受到显著抑制（大于95%）以及在无关抗原存在时无抑制现象所示，该检测方法具有抗原特异性。特应性患者（85名受试者）的检测值显著高于非特应性供者（35名受试者）（1.14 U±1.20对0.01 U±0.02，P＜0.0005），并且与Pharmacia RAST有良好的相关性（P＜0.0005）。REAST和RAST检测的特异性IgE水平与临床症状表现有良好的相关性。
