McGinnes K, Chapman G, Marks R, Penny R
J Immunol Methods. 1986 Jan 22;86(1):7-15. doi: 10.1016/0022-1759(86)90258-9.
A flow cytometric NK assay was developed in which the K562 targets were labelled with the fluorogenic substrate, carboxyfluorescein diacetate (c'FDA). This new assay compared favourably with results obtained using the conventional 51Cr-release assay. c'FDA was not toxic to target cells and did not inhibit lysis. The assay permits the evaluation of various aspects of NK activity such as the activity of NK-enriched, IFN-alpha-activated, and ALG-inhibited populations. The assay can be used in place of 51Cr-release, and has the advantages of being able to directly monitor target cell lysis of reducing overall assay time, and the avoidance of radioisotope usage.
开发了一种流式细胞术NK检测方法，其中用荧光底物羧基荧光素二乙酸酯（c'FDA）标记K562靶细胞。这种新检测方法与使用传统的51Cr释放检测方法得到的结果相比具有优势。c'FDA对靶细胞无毒且不抑制裂解。该检测方法能够评估NK活性的各个方面，如NK富集群体、IFN-α激活群体和ALG抑制群体的活性。该检测方法可替代51Cr释放检测方法，具有能够直接监测靶细胞裂解、减少总体检测时间以及避免使用放射性同位素的优点。
