通过荧光染料羧基荧光素二乙酸酯（CFDA）的靶细胞保留优化细胞毒性测定并与传统的51Cr释放测定进行比较。
Optimization of cytotoxic assay by target cell retention of the fluorescent dye carboxyfluorescein diacetate (CFDA) and comparison with conventional 51CR release assay.
作者信息
Provinciali M, Di Stefano G, Fabris N
机构信息
Gerontology Research Department, Italian National Research Centres on Aging (I.N.R.C.A.), Ancona.
出版信息
J Immunol Methods. 1992 Oct 19;155(1):19-24. doi: 10.1016/0022-1759(92)90266-v.
This paper describes the comparison between a fluorimetric NK assay based on the target cell retention of fluorescent dye carboxyfluorescein diacetate (cFDA) and standard 51Cr release assay. The results provide several suggestions to improve the cytotoxic assay based on the use of the fluorogenic substrate showing that the measurements of cFDA retained by target cells represent a method of evaluating cytotoxicity completely comparable to the 51Cr release assay.
本文描述了基于荧光染料羧基荧光素二乙酸酯（cFDA）在靶细胞中的保留情况的荧光自然杀伤细胞（NK）检测法与标准的51铬释放检测法之间的比较。结果提出了一些改进基于荧光底物使用的细胞毒性检测法的建议，表明对靶细胞中保留的cFDA的测量代表了一种与51铬释放检测法完全可比的评估细胞毒性的方法。
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