Lawal Abiola S, Ogunribido Tobi Z, Fu Yuechi, Adeola Olayiwola, Ajuwon Kolapo M
Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA.
Transl Anim Sci. 2024 Oct 3;8:txae142. doi: 10.1093/tas/txae142. eCollection 2024.
Evidence suggests that nucleotide supplementation in diets improves intestinal development, immune function, and cell growth. Stressful events such as weaning in pigs may increase nucleotide demand, making exogenous supplementation potentially beneficial. This study evaluated the effects of low-protein () diets supplemented with dietary nucleotides on growth performance, postweaning diarrhea (), nutrient digestibility, and blood metabolites. A total of 210 piglets were weaned at 21 d of age, allowing a 3-d adaptation to a common nursery diet. At 24 d, pigs were reweighed (6.02 ± 0.05 kg) and allocated to 5 dietary treatments in a randomized complete block design to give 7 replicates per treatment ( = 6 piglets per replicate). The 5 dietary treatments included (i) a high protein positive control diet () with 24% crude protein (); (ii) a low protein negative control () with 16% CP; (iii) an NC diet with nucleotide supplementation at 1 g/kg (), 3 g/kg (), or 9 g/kg (). Diets were provided ad libitum for 35 d, and weekly feed intake () and body weight () were recorded. Blood samples were collected on day 32 and fecal samples were collected on days 33, 34, and 35 to determine serum metabolites and nutrient digestibility, respectively. Relative to PC, the NC diet had lower overall average daily gain () (343.5 vs. 305.5 g/d), incidence of PWD (2.5 vs. 1.2 diarrhea score), and blood urea nitrogen () (11.3 3.4 mg/dL); ( < 0.05, < 0.05, and < 0.0001, respectively). The nucleotide-supplemented diets, NC01, NC03, and NC09, had comparable (0.05) overall ADG to the PC and decreased ( < 0.0001) BUN. Additionally, NC09 had decreased ( < 0.05) incidence of PWD compared to PC. The apparent total tract digestibility () of nitrogen increased linearly ( < 0.05) with nucleotide supplementation, although reducing CP decreased ( < 0.05) serum glutathione and insulin-like growth factor-1 () concentrations. However, IGF-1 concentration was linearly increased (< 0.05) with nucleotide supplementation. Results suggest that feeding LP diets supplemented with dietary nucleotides after weaning can increase ATTD of nitrogen and protein utilization, reduce the incidence of PWD, and increase serum IGF-1 concentration while partially ameliorating the negative effects of LP diets on growth performance.
有证据表明，日粮中补充核苷酸可改善肠道发育、免疫功能和细胞生长。猪断奶等应激事件可能会增加核苷酸需求，使外源补充可能有益。本研究评估了添加日粮核苷酸的低蛋白（LP）日粮对生长性能、断奶后腹泻（PWD）、养分消化率和血液代谢物的影响。总共210头仔猪在21日龄断奶，使其有3天适应普通保育日粮的时间。在24日龄时，对仔猪重新称重（6.02±0.05千克），并采用随机完全区组设计将其分配到5种日粮处理中，每个处理有7个重复（每个重复6头仔猪）。这5种日粮处理包括：（i）粗蛋白（CP）含量为24%的高蛋白阳性对照日粮（PC）；（ii）CP含量为16%的低蛋白阴性对照日粮（NC）；（iii）分别添加1克/千克（NC01）、3克/千克（NC03）或9克/千克（NC09）核苷酸的NC日粮。日粮自由采食35天，每周记录采食量（FI）和体重（BW）。在第32天采集血样，在第33、34和35天采集粪便样本，分别用于测定血清代谢物和养分消化率。与PC日粮相比，NC日粮的总体平均日增重（ADG）较低（343.5对305.5克/天）、PWD发病率较低（腹泻评分为2.5对1.2）以及血尿素氮（BUN）水平较低（11.3±3.4毫克/分升）；（P<0.05、P<0.05和P<0.0001）。添加核苷酸的日粮NC01、NC03和NC09的总体ADG与PC日粮相当（P>0.05），且BUN降低（P<0.0001）。此外，与PC日粮相比，NC09的PWD发病率降低（P<0.05）。随着核苷酸添加量的增加，氮的表观全肠道消化率（ATTD）呈线性增加（P<0.05），尽管降低CP会降低血清谷胱甘肽和胰岛素样生长因子-1（IGF-1）浓度。然而，随着核苷酸添加量的增加，IGF-1浓度呈线性增加（P<0.05）。结果表明，断奶后饲喂添加日粮核苷酸的LP日粮可提高氮的ATTD和蛋白质利用率，降低PWD发病率，并提高血清IGF-1浓度，同时部分缓解LP日粮对生长性能的负面影响。
