Chakraborty Juhi, Roy Chandrashish, Kalogeropoulou Maria, Mota Carlos, Ghosh Sourabh, Moroni Lorenzo
Department of Textile and Fibre Engineering, Indian Institute of Technology Delhi, New Delhi 110016, India.
Department of Complex Tissue Regeneration, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, 6211 LK Maastricht, the Netherlands.
STAR Protoc. 2024 Dec 20;5(4):103332. doi: 10.1016/j.xpro.2024.103332. Epub 2024 Oct 23.
External magnetic fields can regulate cellular responses. Here, we present a protocol to fabricate magnetic constructs by 4D bioprinting with shape-morphing properties using silk fibroin-gelatin bioinks for articular cartilage regeneration. We illustrate the steps for magnetic bioink formulation, bioprinting, and chondrogenic induction of human bone marrow mesenchymal stem/stromal cells. We detail the steps to actuate the constructs using an external magnetic field and then characterize chondrogenesis. Magnetic field actuation may be helpful for mechanically activating constructs for articular cartilage regeneration. For complete details on the use and execution of this protocol, please refer to Chakraborty et al..
外部磁场可以调节细胞反应。在此，我们展示了一种通过4D生物打印使用丝素蛋白-明胶生物墨水制造具有形状变形特性的磁性构建体的方案，用于关节软骨再生。我们阐述了磁性生物墨水配方、生物打印以及人骨髓间充质干/基质细胞软骨形成诱导的步骤。我们详细说明了使用外部磁场驱动构建体然后表征软骨形成的步骤。磁场驱动可能有助于机械激活用于关节软骨再生的构建体。有关本方案使用和执行的完整详细信息，请参考Chakraborty等人的研究。
